Expression And Clinicopathological Features Correlation Studies Of Adiponectin And Adiponctin Receptor In Papillary Thyroid Carcinoma

Thyroid cancer is one of the most common endocrine malignancy, papillary thyroidcancer (PTC) accounts for approximately60%-80%. During the recent decades, the incidenceof the thyroid cancer has been undergone a worldwide increase and has the serious in humanbody health.Adipocytokines have be closely related to the development of tumor,especiallyadiponectin and leptin as the Yin-Yang adipokines have great significance for molecularmechanism of cancer. In1955, Schere and others found that the fat factor, in1999, Arita,such as it is called adiponectin.Many studies have shown consistently that Adiponectinand Adiponectin receptors(Adipo R1, R2) is associated with some types of cancer, breastcancer, colorectal cancer and endometrial cancer. But now there is no obviously evidence thathow does Adiponectin work in PTC. So our study’s purpose is to examine the expressionand clinicopathological features correlation of Adiponectin and AdipoR receptors in PTCtissue.And we study the expression of Adiponectin and AdipoR and the affection ofAdiponectin on growth and migration in PTC K1cell line,on for offering a new mentality forthe molecular mechanism and prognosis of PTC.Objective Our purpose is that the expression and the role of Adiponectin on growthand migration in PTC, offering evidence of PTC’s molecular mechanism, therapy andprognosis.Methods Our study has two parts. The frist part is that the expression of Adiponectinamd AdipoR was detected by immunohistochemistry (IHC) in PTC tissues and thyroidadenoma tissues, and analyse whether the expression of Adiponectin amd AdipoR areassociation with clinical features such as Age, weight, menopausal status, tumor size, lymphnode metastasis and so on. The second parts is that we the expression of Adiponectin and AdipoR was detected by Western Blot on the level of in PTC K1cell line. We measuredeffect of Adiponectin on PTC K1cell growth and migration using CCK-8assary and scrachtest.Result By the IHC,we researched that the positive expression rates of Adiponectinwere94.4%(72/78) and66.74%(4/12) in PTC and thyroid adenoma tissues respectively. Thedifference between the two groups was significant (P<0.05). In papillary thyroid carcinomatissues,the preoperative body weight of the both adiponectin positive expression groups withthe negative expression groups was obviousiy different. The differences were significant(P＜0.05), Adiponectin expression positive than negative expression in patients with preoperativehigh weight. The rates of positive expression adiponectin receptor1and adiponectin receptor2were40.1%and38.7%, both express correlation, between expression or not together. Inpapillary thyroid carcinoma tissues,the age and menopause of the both adiponectin receptor2positive expression groups with the negative expression groups was obviousiy different.Thedifferences were significant(P＜0.05), menopause and age large AdipoR2positive expressionor high expression. No significant associations were observed between adiponectin receptor1and clinicopathological variables. The OBR and AdipoR2comparison was statisticallysignificant, both positive or high expression of OBR and AdipoR2low or negative expression.By the Weatern Blot, we detected the expression of Adiponectin and AdipoR on the level ofprotein in PTC K1cells line. The result of CCK-8and cell wound scratch assay showed theability of K1cells to proliferation and migration was reduced when treated with Adiponectin.Conclusion Adiponectin and its receptors are expressed in the PTC and K1cells line,Adiponectin is associated with preoperative weight, AdipoR2is associated with age andmenopausal status, K1cell proliferation and invasive ability lower are Adiponectin processing,Adiponectin and its receptors may play a role in PTC development.