Protective Effects Of1,25-Dihydroxyvitamind₃to Non-Alcoholic Fatty Liver Hepatitis In Rats

Objective:To investigate the protective effects of1,25-dihydroxyvitamin D3to non-alcoholic fatty liver hepatitis induced by choline-deficient L-amino acid-defined (CDAA)in rats.Methods:Healthy male Wistar rats (SPF) were randomly divided into control group, model group and1,25(OH)2D3high, medium and low doses groups, with ten rats in each group. We applied CDCC diet inducing method to establish the models of non-alcoholic fatty liver hepatitis. Among them, the rats in both model group and the1,25(OH)2D3intervention group were fed with CDAA diet, whereas the rats in control group were fed with acholine-sufficient L-amino acid-defined (CSAA). The rats in groups of high, medium and low doses of1,25(OH)2D3were given1,25(OH)2D3hby intraperitoneal injection, concentrations were10,5, lug/ml/kg, respectively. The rats in both control group and model group received injection of the same amount of physiological saline by intraperitoneal injection, with two times a week. At the intervention of twelve weeks, blood samples were collected from abdominal aorta and liver tissue specimens were obtained from five rats from each group in this study. We applied the conventional method to determine the serum biochemical indexes, as well as observed the pathological changes of hepatic tissue by performing HE staining method.Results:The values of serum alanine arninotransferase (ALT) and aspartate aminotransferase (AST) from the medium does of1,25(OH)2D3group were significantly lower than the level of model group (P<0.01). The liver tissue revealed diffuse fatty degeneration with the center of central vein, a little spotty necrosis and fibrous tissue hyperplasia were shown in model group, whereas different degree of improvement of these histopathologic changes were observed in various doses of1,25(OH)2D3model groups. The effect of medium dose of1,25(OH)2D3model group was the best; lesions were reduced in the low dose group; no significant difference was found between high dose group and the control group in this study. Conclusions:This study showed that the medium dose of1,25-dihydroxyvitamin D3possessed significant protective effects to the non-alcoholic fatty liver hepatitis in rats induced by choline-deficient L-amino acid-defined (CDAA).