The Development Of Screening Kits For Diagnosis Of AIDS Common Opportunity Infection Of Fungal

AIDS can reduce immunodeficiency and result in a number of human pathogenssusceptible to pathogenic fungi. Statistics show that90percent of people die fromopportunistic infections of AIDS. Fungal infections in AIDS patients with blood arethe most common opportunistic infections. There are a high morbidity and mortality.The incidence of fungal opportunistic infections of AIDS were common. But we lackthe fast and effective detection products. Molecular diagnostic is a fast, strongspecificity and high sensitivity method. It have the ability which can detect theinfection in the early. But traditional molecular biology techniques for detectingdefects has the lower flux. But gene chip technology solve this problem due to its highthroughput detection.In recent years, deep fungal infection incidence is increasing year by year alongthe widely of broad-spectrum antibiotics, hormones and immunosuppressants. Themost common pathogens were Candida albicans, Aspergillus fumigates andCryptococcus performance. The rare Penicillium and yeah Pneumocystis fungipathogenic microorganisms also showed an increasing trend. This study of the currentsituation and status of HIV common fungal infection of the AIDS epidemicopportunity to analyze. This study select five common opportunistic fungal infectionfor detecting objects. They were Candida albicans, Aspergillus fumigates,Cryptococcus performance, Penicillium and yeah Pneumocystis. The work report isbelow.Purpose： To develop a new visual gene chips which can detect several fungalopportunistic pathogens: Penicillium, Cryptococcus performance, Candida albicans,Aspergillus fumigates, yeah Pneumocystis.For clinical provides a fast and effectivemethod.Method: Based on the hybridization and visual color theory, sap6, cyp51A gene, lac1gene, gas gene and18s genes were selected to design and screening of five kinds offungus-specific primers and probes. We use primers labeled with biotin to runmultiplex PCR amplification. Then wo use the amplified product and a pre-fixed onthe slide aldehyde five fungal specific probes to conduct hybridization reactions. Theprobe signal is rendered visible with Tyrannidae signal amplification which use a combination of quantum dots labeled silver staining color enhancement techniques.We can specifically detect five opportunistic fungal infections through analysis.Result: The study ultimately selected five pairs of specific primers and five specificprobes to optimize the multiplex PCR system, the hybridization reaction andvisualization color conditions. We also investigated detection performance microarray.The gene chip detection sensitivity can be achieved103PFU/mL.Conclusion: The gene chip has a high sensitivity, low cost, etc. So it can be used as anew method for the clinical diagnosis of fungal infection and provides a simple, rapid,sensitive method for the diagnosis of fungal opportunistic infections in patients withHIV.