| Background:Neurotrophins (NTs) is a kind of important family of neurotrophic factor, playsan important role in maintaining the survival and regeneration of nerve cells.Neurotrophin-3(NT-3) is the third member, with a wide range of neurotrophicactivity. Spinal ganglion sensory ganglion, containing many false unipolar neuronscell body and parallel arrangement of nerve fiber bundle, is mainly responsible for thefeeling of limb function.Lumbar spinal ganglion injury repair, is currently the difficultmedicine problem to be solved.Objective:By severing rat sciatic nerve to establish lumbar spinal ganglion injury model, toillustrate the significance of experimental nerve nutrients-3(NT-3) in promotingneural functional recovery after lumbar spinal ganglion injury and to providetheoretical basis for clinical treatment of lumbar spinal ganglion injury.Methods:Fifty-four SD rats were used,male and female unlimited, randomly divided intothree groups: Sham-operated group, the NT-3treatment group and control group,Sham-operated group only exposed sciatic nerve;The NT-3treatment group off thesciatic nerve in the piriform10mm and inject1ml NT-3(10ug/m1) every daysubcutaneously. The Control group inject subcutaneously every day at the same volume of physiological saline treatment group.In postoperative4week, Observedthe general situation of rats，took the rat lumbar spinal ganglion into austenite staining,HE staining, and to observe the pathological morphological changes of spinalganglion neurons, local inflammation and the Bcl-2, Bax expression positive rate, todetect the apoptosis of spinal ganglion.Results:General observation: the rat sciatic nerve from patrol, lower operation arepresent drooping ankle deformity, limping gait movement dysfunction, with timesuccessively appeared toes swelling, scattered, dry skin, part of the fur shedding,ulcers, autophagy toes, muscle atrophy and other absence management phenomenon,the NT-3treatment group was slightly slightly above phenomena.The NT-3treatmentgroups see the proximal nerve new diameter than the control group is bulky.Austenite staining: After sciatic nerve cut off, the austenite was dissolved todisappearance, number decreased significantly in the Control group, and spinalganglion neurons got a significantly lighter color, cell body swelling, contour owedclear, number of nerve cells decreased significantly.In the NT-3treatment group thedyeings of spinal ganglion neurons were milder compared with control group, and themorphological structure of spinal ganglion neurons were basic normal, and thedecrease in the number of nerve cells is not obvious.HE dyeing observation: In the Control group the number of postoperativespinal ganglion neurons decreased obviously, the cell body appeared edema, vacuolesdegeneration, the austenite dissolved with lighter dyeing, nucleus pycnosis, and therewere typical cell apoptosis.The NT-3treatment groups spinal ganglion neurons cellbody edema, degeneration and apoptosis got a lighter degree than the controlgroup,and the neuron dyeing was deeper.The Bcl-2, Bax immunohistochemical detection: Immunohistochemical Bcl-2, Bax positive staining for tan. Had not seen or only a small number of positivecells in control group.Control group dyeing was deeper, the Bcl-2, Bax positive cellswas significantly higher than sham-operated group (P <0.05), which showed that damage was aggravating.The Bcl-2, Bax positive cells expression of the NT-3treatment group significantly lower than control group (P <0.05),which indicated thatthe NT-3of ganglion protection effect was obvious.Conclusion:Sciatic nerve severed can cause lumbar spinal ganglion injury, the mainlymanner of death of spinal ganglion neurons is apoptosis;NT-3plays an importantnutritional role on maintaining the spinal ganglion neurons survive and inhibiting cellapoptosis. |
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