The Alterations Of Microglia And Vasculature In Developing Tg2576Transgenic Mouse

AD is a kind of progressive memory loss, cognitive dysfunction, life skills and mental declineabnormal behavior as the main clinical features of the central nervous system degenerative encephalopathy.its incidence is increasing year by year, has seriously affected the normal life of the elderly and physicallyhealth, which has become a world problem. Microglia is the most important immune defense of CNS.Neurodegenerative diseases such as Alzheimer’s disease (AD) causes microglia cell morphology andchange the number. Microglial cells release neurotrophic factors and anti-inflammatory cytokines andsynaptic plasticity, but the nerve inflammation can cause persistent activation of microglia releasepro-inflammatory cytokines, which increased the inflammatory response caused nerve damage. The AD-specific β-amyloid can cause cerebral amyloid angiopathy (CAA) is generated, further damage to thebrain blood vessels. We use the wild type mouse (WT) and Sweden pedigree β-amyloid precursor protein(APPswe) transgenic mice (Tg2576) to explore both microglial proliferation of mouse hippocampal CA1development and vascular changes in the law.Objective: Our aim is to study the alterations in microglia and vasculature in the developinghippocampus of Tg2576transgenic mice.Methods: Tg2576transgenic mouse from postnatal day30to postnatal day360were used asAlzheimer’s disease (AD) model. Immunohistochemistry, TdT-mediated dUTP Nick-End Labeling(TUNEL), Transmission electron microscopy and RT-PCR were carried out analyze the change in braintissue.Results: From P180, the density of positive cells in CA1area of AD model of transgenic mouse weresignificantly higher than wild type mouse (P＜0.01). The vasculature (volumetric density) of transgenicmouse at P360were significantly less than in the wild-type mouse (P <0.01). RT-PCR result also showedthat the activity of microglia was enhanced in AD model. Conclusion: The onset and development ofAlzheimer disease is correlated to the alterations microglia and of vasculature in hippocampus.