| Objective: To analyze the expression of miR-200c in the gastric cancer andinvestigate the role of miR-200c in the gastric cancer cells.Methods: The expression of miR-200c was tested by ISH. DNMT3B3’UTR-luciferase vector was constructed and luciferase reporter gene assay wasemployed to examine the effect of miR-200c on luciferase activity. MGC-803cellswere transfected with miR-200c mimics, and next Western blot was performed todetect the expressions of DNMT3B protein. Cell growth was evaluated by MTT.Results: miR-200c was down-regulated in gastric cancer tissues. miR-200c levelswere significantly correlated with the clinical stage (P=0.003), lymph node metastasis(P=0.004) in gastric cancer patients. Luciferase reporter vector system confirmed thatDNMT3B was a target gene of miR-200c. Western blot showed that the expression ofDNMT3B protein was inhibited by restored miR-200c or si-DNMT3B in MGC803cells. miR-200c could inhibit gastric cancer cell proliferation identified by MTTassay(P<0.05).Conclusions:1. The expression level of miR-200c was down-regulated in gastric cancer and had aclose correlation to lymph node metastasis and advanced clinical stage.2. miR-200c can inhibit gastric cancer cell growth by inhibiting the expression ofDNMT3B. |
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