| Objective: Rehabilitation training is one of the effective treatmentmeasures to promote the recovery of neural functional after ischemicstroke. Many empirical studies have made sure that early rehabilitationtraining can obviously improve neurologic deficits symptoms of thepatient after ischemic stroke and improve the quality of the patient’s dailylife. Numerous studies have shown that the activation of cAMP-PKA-pCREB signal pathway can enhances regeneration of neurons axonafter injury. In order to explore the two aspects whether have inextricablylinked, the text regards cAMP-PKA-pCREB signal pathway as thebreakthrough point, through tested the concentration of cAMP and proteinkinase A, tested the expression of protein kinase Catalytic subunit andpCREB and evaluated the recovery of motor function by balance beam testto investigate the role of cAMP-PKA-pCREB signal pathway inrehabilitation training for the motor functional recovery in rats afterischemic stroke and to provided further new supports for the theory ofrehabilitation training.Methods: Choose the healthy adult male Sprague-Dawley rats, andthen established the middle cerebral artery occlusion model (MCAO) by modified Longa nylon method, extract the line after occlusion2hours.The successful MACO rats were selected and randomly assigned to fourgroups: the natural recovery group without any special training，the naturalrecovery group with Rp-cAMP, the rehabilitation training group and therehabilitation training group with Rp-cAMP. After intracerebroventricularinjection of Rp-cAMP, we immediately began to establish the MACOmodel. Meanwhile to designed a sham operation group. The rehabilitationtraining rats were trained by balance beam, bar rotating and rollingexercises initiated48h after surgery. The concentration of cAMP and PKAwere determined by enzyme-linked immunosorbent assay (ELISA), PKAcand pCREB were tested by Western Blot and used immunohistochemicalmethod to detected the location of PKAc, pCREB and GAP-43and to dohemi-quantitatively analysis for the expression of GAP-43. Motor functionwas assessed by balance beam test.Results:(1) The results of motor function score: the scores of naturalrecovery group rats was significantly lower than that in natural recoverygroup with Rp-cAMP, rehabilitation training group rats significantly lowerthan natural recovery group, also are lower than the rehabilitation traininggroup with Rp–cAMP, especially at14th days after surgery;(2) Theconcentration of cAMP, PKA and the expression of PKAc, pCREBshowed significantly higher in natural recovery group than in naturalrecovery group with Rp-cAMP, rehabilitation training group rats aresignificantly higher than natural recovery group rats and also higher thanthe rehabilitation training group with Rp–cAMP, especially at14th daysafter surgery.(3)The results of GAP-43protein expression showed thatnatural recovery group rats was significantly higher than that in naturalrecovery group with Rp-cAMP, rehabilitation training group rats significantly higher than natural recovery group, also are higher than therehabilitation training group with Rp–cAMP, especially at14th days aftersurgeryConclusion:(1)The concentration of cAMP and PKA were increasedafter cerebral tissue ischemia and the expression of GAP-43protein alsoincrease.(2)Rehabilitation training can significantly enhanced theconcentration of cAMP and PKA, the expression of PKAc and pCREB inthe brain tissue after cerebral ischemia in rats, and significantly increasedthe expression of GAP-43protein.(3) The MACO rats withIntracerebroventricular injection of Rp-cAMP can obviously inhibit theexpression of PKA, PKAc and pCREB protein, and can obviously inhibitthe improving of Rehabilitation training.(4) Rehabilitation training cansignificantly improving the recovery of motor function after MACO inrats.(5) The MACO rats with Intracerebroventricular injection ofRp-cAMP can obviously inhibit the recovery of motor function in naturalgroup rats after cerebral ischemia. According to the above research finding,the activation of cAMP-PKA-pCREB signaling pathway to promotingthe axonal regeneration may be related to the natural recovery of motorfunction, and may be one of the important mechanisms of rehabilitationtraining for the improvement of motor function recovery after cerebralischemia rats. |
