DDP Inhibits The Proliferation Of Hela Cell Line Via STAT1/c-myc Pathway

Objective:To investigate the role of STAT1gene in the pathogenesis of cervical cancer to chemotherapy, explored the genes for targeted small interfering RNA (siRNA) could effectively reverse the Hela cells to cisplatin (DDP) to chemotherapy.Method:The protein expression of STAT1in the Hela cells which treated with concentration gradient of DDP was detected by Western blot assay; Western blot assay was used to validate whether the STAT1gene was silenced. The influence of siRNA on cellular sensitivity to DDP and cell proliferation activity was detected by MTT assay and BrdU assay. The expression of c-Myc was detected by Western blot assay in the Hela cells treated with siRNA and DDP.Results:The expression of STAT1in Hela cells at DDP with the concentration gradient of rising DDP when concentrations of5mg/1, increased expression of STAT11.5times, DDP when concentrations of10mg/l, increased expression of STAT1twice times. STAT1-siRNA can effectively reduce the STAT1in Hela cell proteins, promotes cell growth in the proliferation, and the expression of c-Myc; DDP can inhibit growth and proliferation of Hela cells, down-regulate the expression of c-Myc, and combined the treatment of DDP and STAT1-siRNA can rescue inhibition of DDP on cell growth and proliferation, and down-regulation of the expression of c-Myc.Conclusion:The expression of STAT1associated with cisplatin sensitivity in carcinoma of the cervix; STAT1-siRNA inhibits the expression of STAT1and effective response of DDP resistance of Hela cells and expression of c-Myc.This paper concludes16graphs,8tables and45references.