Differential Regμlation Of Host Genes Including Hepatic Fatty Acid Synthase In HBV-transgenic Mice

In human, chronic hepatitis B virus (CHB) infection is the mostcommon causes of liver disease, and Hepatitis B virus (HBV) isconsidered to be a global health care issue. In order to increase knowledgeof hepatitis B pathogenesis, as well as discover potential novel moleculesfor anti-HBV therapy, mass spectrometry (MS) and iTRAQ were employed.A group of Liver samples were from HBV-transgenic (Tg) mice, anothergroup of Liver samples were from wild-type (WT) mice (correspondingbackground normal mice). In total,68proteins were analyzed fordifferential expression in the two types of samples. We used real-time PCR,Western blotting and IHC to further validate several differentiallyexpressed proteins. Furthermore, We found a relationship between HBVreplication and fatty acid synthase (FASN). FASN silencing suppressedviral replication in HepG2.2.15cells through the IFN signaling pathway.Thus, FASN may be a new therapeutic target for HBV replication.