Amphotericin B Suppresses The Migration And Invasion Of Esophageal Carcinoma Eca109Cells In Hypoxicmicroenvironment By Down Regulating The Activity Of The HIF-1α

Objective: To research the impact of Eca109cell invasion andmigration after treat with amphotericin B (AmB) under hypoxicconditions， and explore its probable molecular mechanisms.Methods: Treated the Eca109cells in logarithmic growth phase withthe AmB at a dose of0,1.25,2.5and5μg/mL respectively, continue toculture the cells in the three gas incubator(37℃,3%O2,5%CO2,92%N2)for24h. Cell scratch test was used to detect the ability of the cellmigration；Transwell invasion assay was used to detect the ability of thecell invasion；Real-time PCR and Western blot were used to detect themRNA levels and the protein levels of HIF-1α, E-cadherin and MMP-2respectively.Results: Compared with control group (0μg/mL), the cell migrationdistance and the number of invading cells decreased (P<0.05) after treatingwith each dose of AmB(0,1.25,2.5,5μg/mL); the levels of mRNA andprotein expressions of MMP-2were decreased significantly after treating with AmB(P＜0.05); the levels of mRNA and protein expressions ofE-cadherin were increased obviously after treating with AmB(P＜0.05);protein expressions of HIF-1α were decreased significantly in controlgroup than in treating with AmB group in Eca109cell line (P＜0.05), whilethe mRNA levels of HIF-1α had no significant change(P>0.05).Conclusion: Eca019cells migration and invasion abilities will declineafter treat with AmB under hypoxic conditions，the probable molecularmechanism is via the regulating on HIF-1α，E-cadherin and MMP-2.