Construction And Application Of Novel Electrochemical Sensor Based On PTC-NH₂Functionalized Nanomaterials

Conventional analysis of biological samples owns complexpretreatment, a higher cost and a longer analysis time. Thus, there is greatdemand in development new analytical methods for simple, rapid andsensitive detection of active substances in samples. Recently,electrochemical sensing technology has been widely used in the biologicalanalysis and clinical diagnostics due to its high selectivity, high sensitivityand simple operation. Preparation of excellent modified material playscrucial role in electrochemical sensor construction successfully.Nano-materials have captured worldwide interest in biosensor constructiondue to its excellent biocompatibility, outstanding electrical conductivity andhigh surface-to-volume ratio. Thus this work aims to construct newbiosensors for simple, rapid and sensitive detection of ACOP, H2O2andJAR in samples based on a derivative of3,4,9,10-perylenetetracarboxylicdianhydride which is abbreviated to PTC-NH2. The detail contents are asfollows： 1. Electrochemical Sensor for Sensitive Detection ofAcetamino Phenol Based on Novel Multi-walled CarbonNanotubes-Derived Organic-Inorganic MaterialA novel organic-inorganic material (PNFCTs) composed ofmulti-walled carbon nanotubes (MWNTs) and PTC-NH2was preparedthrough an amidation reaction between the amine groups attached toPTC-NH2and the available carboxylic acid groups of MWNTs. Then,PNFCTs was coated onto the surface of the glassy carbon electrode (GCE) toform porous organic conducting polymer films (PNFCTs/GCE), whichcould increase the loading of ACOP efficiently. Finally, gold nanoparticles(GNPs) were attached to the electrode surface through electrodepositingmethod, which obtained GNPs/PNFCTs/GCE electrode. The results showedthat the GNPs/PNFCTs/GCE exhibited excellent electrocatalytic activity toACOP. The wide liner range and detection limit was0.3to575μmol·L-1and0.1μmol·L-1, respectively. The sensor shows great promise for simple,sensitive, and selective detection ACOP.2. Protected Prussian Blue-nanocomposites hybrid film: A NovelNon-enzymatic Hydrogen Peroxide SensorPrussian blue (PB) has shown strong catalytic property for thereduction of hydrogen peroxide (H2O2), but the application in biosensorconstruction is limited for its instability. To improve stability of PB, a novelkind of nanocomposites material (PTBFGs) was prepared. It composed ofgraphene oxide (GO) as a carrier, electroactive PB and PTC-NH2. First of all, PB was synthesized on the surface of GO. Then PTC-NH2was linked on thesurface of PB-GO which serves as not only a barrier of PB leakage but alsoelectronic transport-accelerator due to its unique electrochemical properties.This organic-inorganic nanocomposite film demonstrates extraordinaryproperties like being stable, easy to form a porous film and transferelectrons. The results showed that the modified electrode own outstandingcycling stability. The proposed non-enzymatic sensor possesses highelectrocatalytic activity towards the reduction of H2O2and the amperometricresponses shows a linear dependence on the concentration in a range of0.5to544.5μmol·L-1and a low detection limit of0.07μmol·L-1. Therefore, thiswork provides novel nanocomposites, which own unique characteristics andhold numerous applications in the fields of sensors and electrocatalysis.3. Electrochemical Sensor for Sensitive Detection Cancer Cell Based onNovel Graphene Oxide-Derived Organic-Inorganic MaterialA novel organic-inorganic nanocomposite (GOPs) composed of GOand PTC-NH2was prepared through an amidation reaction between theamine groups attached to PTC-NH2and the available carboxylic acid groupsof GO. Then, GOPs was coated onto the surface of the GCE to form porousorganic conducting polymer films (GOPs/GCE). After GNPs were attachedto the electrode due to a great amount of amine groups of PTC-NH2,sambucus nigra agglutinins (SNA), binding specifically with sialylatedglycans, was covalently immobilized on the GNPs. The capture of JAR was according to the specific binding between SNA and sialylated glycans onthe surface of JAR cell. We choose [Fe(CN)36]-/4-as redox probe. Mainexperimental conditions parameters were investigated. Our results revealedthat SNA based cell biosensor showed a wide linear range (1.1×103~5.5×105cell·mL-1) and a low detection limit of350cell·mL-1. In this study, itis the first time that GOPs were synthesized and applied for constructioncancer cell biosensor. This study provides a good reference for thedevelopment of simple, rapid, sensitive new analytical method for rapiddetection of tumor cells JAR.