Effect Of Acupuncture On Islet Beta Cell Apoptosis In Type2Diabetes Research From The Endoplasmic Reticulum Pathway

Purpose:Studying the role of acupuncture on islet beta cell apoptosis and the relationship between the endoplasmic reticulum stress, then discussing the cellular and molecular mechanisms, having great theoretical significance for prevention and treatment of type2diabetes effectively.Method:We according to the pathogenic factors of type2diabetes, we successful development of type2diabetic rats model, which materials as experiment object, monitoring rats blood indexes. Using real-time quantitative PCR jade-like stone light observation rat pancreas after acupuncture of caspase-12, Bax, microplate method using observed in the cytoplasm of Ca2+changing. To study acupuncture by endoplasmic reticulum stress means to improve the molecular mechanisms of apoptosis.Results:After acupuncture treatment of rats of FPG, FINS, GHbAlc indicators have improved significantly, Combined with clinical observation fully illustrates the acupuncture can reduce FPG in type2diabetes and to improve the symptoms of diabetes. Acupuncture treatment of caspase-12indicators in the cells of rats to be less than model group, one of intradermal needle group caspase-12indexes are significantly lower than other treatment group, has reached the level of the normal group, fully shows the acupuncture therapy can inhibit caspase activation, reducing the generation of ERS. In pancreatic cells in rats after acupuncture, the number of Bel-2significantly increased, enhanced cell antiapoptotic functions, at the same time reduce the Bax conformational change (the experimental data show that rats decreased significantly, the number of Bax after acupuncture has reached the level of normal rats), to maintain the integrity of the endoplasmic reticulum, stop the outflow of the intracellular Ca2+, less that the number of Ca2+in the cytoplasm, which reduces the ERS. These results are fully shows the acupuncture therapy in patients with type2diabetes, can inhibit the occurrence of ERS way, prevent abnormal apoptosis process of islet beta cells, maintain the normal physiological function of insulin, eventually to improve FPG.