| Objective: To investigate the effects of DADS on invasion and migration in humangastric cancer MGC803cells after silencing cathepsin D, further to clarify themolecular mechanisms that DADS inhibits migration and invasion of gastric cancer.Methods: Firstly, Cath-D siRNA was transfected into human gastric cancerMGC803cells with lipofectamine transfection methods, then MGC803recombinantcell line were formed; MGC803recombinant cell line and human MGC803gastriccancer cell line were treated by DADS (30mg/L); The mRNA and protein expressionlevel of Cath-D were determined by RT-PCR and Western blot respectively before andafter treated by DADS in three cell lines (Cath-D siRNA interference group, negativecontrol group and blank control group); Scratch test and transwell assays examinedmigration and invasion respectively before and after treated by DADS in three celllines (Cath-D siRNA interference group, negative control group and blank controlgroup).Results: After Cath-D was interfered by siRNA, the mRNA and protein expressionlevel of Cath-D were significantly decreased (P<0.05), scar distance was wided(P<0.05), the number of cancer cells which passed through the Matrigel coatedmembrane was decreased (P<0.05). Human gastric carcinoma MGC803cells exposedto DADS (30mg/L) for24h, the mRNA and protein expression level of Cath-D weresignificantly decreased (P<0.05), scar distance was wided (P<0.05), the number ofcancer cells which got through the Matrigel coated membrane was decreased (P<0.05).And, in the Cath-D siRNA interference group treated with DADS, the mRNA andprotein expression level of Cath-D were decreased most significantly (P<0.05), scardistance was widest (P<0.05), the number of cancer cells which got through the Matrigel coated membrane was least (P<0.05).Conclusion:1.Cath-D siRNA can inhibit migration and invasion of human gastric cancer MGC803cells.2.Silenced Cath-D increases the inhibiton effects of DADS on migration and invasionin human gastric cancer MGC803cells. |
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