Protective Effect Of Rosiglitazone On The Acute Lung Injury Induced By Sepsis And The Influence On The Endothelial Growth Factor Level

Objective:Establishing a scientific and reproducible animal model ofsepsis-induced lung injury.To investigate the protective effect of rosiglitazone onthe acute lung injury induced by sepsis,and to observe the influence on Vascularendothelial growth factor.Methods: In this study,seventy-two Sprague-Dawley (SD)rats were equallyrandomly divided into three groups:sham operation group (Sham group), sepsis group(Sepsis group), and rosiglitazone-treated group (ROSI group).In which,Sepsis groupand ROSI group were operated by modified cecal ligation and puncture.ROSI groupwere treated with rosiglitazone4mg.kg-1.d-1after operation.At12,24h after operationarterial partial pressure of oxygen(PaO2);wet-to-dry weight ratio;the pathologicalchanges of lung tissue detected by HE staining were measured;the vascularendothelial growth factor,nuclear factor-kappa-B，Interleukin6in blood serum weremeasured by enzyme linked immunosorbent assay (ELISA) and vascular endothelialgrowth factor mRNA level of lung tissue were assayed by reverse transcriptionpolymerase chain reaction（RT-PCR）.Results:Compared with sepsis group,arterial partial pressure of oxygen in ROSIgroup were significantly increased at any point of time;The ratio of W/D was lowerthan that in sepsis group (P <0.01). The levels of serum NF-κB、IL-6in ROSIgroup were significantly decreased compared with Sepsis group (P <0.01);the levelsof serum VEGF were lower,while the levels in lung tissue were higher than those insepsis group.Conclusion:Rosiglitazone could alleviate the extent of acute lung injury induced bysepsis,and this might be associated with decreasing the levels of serum VEGF,and increasing the VEGF of lung tissue.