Investigate The Protective Effects And Mechanism Of Rhizoma Gastrodia On Radiation Damage In Rat Spleen

ObjectiveTo investigate the mechanism and protective effect of Rhizoma Gastrodiaon spleen in rats with radiation injury.MethodsThe54healthy male SD rats, were divided randomly into3groups, controlgroup, irradiation group, the experimental group. each group have18rats.Controlgroup:Don’t do the irradiation experiment,2days before to7days after theexperiment continue to fed with physiological saline2ml/10g orally,1times/day;irradiation group: the whole body irradiation with60Co gamma ray,2days before to7days after the irradiation continue to fed with physiological saline2ml/10g orally,1times/day, experimental group: the whole body irradiation with60Co gamma ray,2days before to7days after the irradiation continue to fed with gastrodin injection2ml/10g orally,1times/day.6Rats in each group after irradiation1、3、7days weresacrificed and take the spleen,through the colorimetric method to detect MDAcontent, SOD activity, Caspase-3activity, TUNEL detect the cell apoptosis、use thewestern blot method to detect the expression of Bcl-2, Survivin protein And observedthe spleen tissue pathological changes under the light microscope.ResultsThe change of the content of MDA and the SOD activity in each group Show:InIrradiation group and experimental group the content of MDA were higher than thecontrol group, the activity of SOD was lower than that of the controlgroup,P<0.05.the content of MDA irradiation group was higher than that in the experimental group, the SOD activity is lower than that in the experimental group,P<0.05; reflect the injury degree of cells the experimental group relaxed more thanthe irradiated group.Each time, the control group1D,3D,7D, MDA content and SODactivity had no significant difference, P>0.05;Irradiation group and experimentalgroup MDA content is3d>7d>1d，, the activity of SOD is3d <7d <1d, P<0.05。The changes of spleen tissue cell apoptosis related factor in rats Show： InIrradiation group and experimental group, cell apoptosis rate、 Caspase-3enzymeactivity was higher than that of control group, P<0.05; The apoptosis rate andCaspase-3enzyme activity of irradiation group was higher than that in theexperimental group, the expression of Bcl-2and Survivin protein was lower than theexperimental group, P<0.05; The apoptosis rate of the experimental group was lowertips. Each time, there was no significant difference of the index of1D,3D,7d group,P>0.05; In Irradiation group and the experimental group, the apoptosis rate andCaspase-3enzyme activity is1d>3d>7d，Bcl-2and Survivin protein expression is1d <3d <7d，P<0.05。The rats spleen biopsy showed: the control group seen in low power can see thecomplete capsule, there is well-demarcated between the red and white pulp,haveclear trabecular structure,Vascular clearly, germinal center Obvious,At highmagnification, Cells are arranged in compact and orderly is seen,Compared with thecontrol group,irradiation group,the experimental group spleen capsule integrity, cellsare sparse, the white pulp and marginal zone can be seen in the large number ofapoptotic cells, the morphology of cell apoptosis can be seen, extravasated red cells.Compared with the irradiated group, the experimental group each time the cellsarranged more closely, relatively small apoptosis cells, exudation of RBC less.Gastrodia elata reduces tissue radiation damage to a certain extent; group, controlgroup1D,3D,7d were no significant difference of less than3D, the apoptotic cellsirradiated group1D,exudation of RBC is relatively light,7d than3D slightly recovery,apoptotic cells than3D1D in experimental group was restored, exudation of RBC is relatively light,7d than3D slightly recovery.ConclusionGastrodia elata can reduce the content of MDA,increase the activity ofSOD,promot Survivin,Bcl-2protein expression,reduce the Caspase-3activity,andreduce the cell apoptosis,has a protective effect on radiation injury of spleen.