Nir Heptamethine Complex Dyes PZ-1009Preliminary Exploration In Ovarian Cancer Imaging

The introduction of the single photon emission tomography has morethan30years.The radionuclide imaging technology already cannot bementioned in the same breath.Cumulative of talents and update of machineshave shorted the distance between the developed countries andus.SPECT/CT based on a scanning of a patient to obtain the functionalimaging and anatomical imaging which has been used commonly inworld.For lesion location and disease diagnosis, it has many advantages.Fluorescence imaging, especially the near-infrared fluorescence imagingcould non-invasive and repeated to observe molecular events in the life.Researchers hope that more advanced technologies were applied to clinicaldevelopment, for example the combination of nuclide and fluorescence inthe molecular imaging, and the special method could be called multimodalmolecular imaging：a targeted probe which has a variety of imagingfunctions be injected into the body, then using some instruments to obtaindifferent informations on the same site at the same time. More conducive toget location of lesion and its nature. Today is also the hot trend of medicalimaging. Early Xiaojian Y et al found that the near-infrared heptamethinephthalocyanine dyes MHI-148has tumor targeting in a broad spectrum oftumor[1], Professor Pan used the DOTA to conjugate with the dye, then thechelating agent combined with a radionuclide-64Cu. So that they couldachieve the dual mode imaging include near-infrared fluorescence andpositron emission tomography imaging of breast cancer because the perfectcompound[2]. Lately, they connected MHI-148with another chelating agent(named PZ-1009)for looking forward to the NIRF combined withSPECT/CT.The members of our team have used incubation method and a confocalmicroscope to observe the compound’s distribution in ovarian cancer cellsfirstly, injected the compound through intravenous after established tumormodel in nude mice secondly. Images collected at different time pointsusing in vivo imaging instrument. Mice were killed which have thestrongest fluorescence intensity at one time point after injection. At last，thetumors and vital organs be imaged what they got at6h time point. Resultsshowed that: ovarian cancer cell cytoplasm was significant staining.Afterinjection of1h there was a strong fluorescence signal in tumors. Whenmeasured using a imaging software the maximum signal strength is at6h,and there still remained visible fluorescence signal until48hs. Thefluorescence signal intensity of tumor at any time point were higher thanthe control part(p＜0.05). In conclusion：even though near-infrared dyes MHI-148conjugated with other substance was still having the obvioustumor targeting, has laid a good foundation for the preparation ofmulti-modal probe.