Inhibitory Effect Of Cetuximab Combined With Paclitaxel On Nasopharyngeal Carcinoma Cell Line CNE-1and Its Molecular Mechanism

Objective: To study the effects of Cetuximab combined with paclitaxelon proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-1and its molecular mechanism.Methods: Nasopharyngeal carcinoma cell line CNE-1were culturedand divided into vacuity contrast group, cetuximab group, paclitaxel groupand combination group. The inhibitory rate was detected by MTT, theapoptosis rate was analyzed by flow cytometry, and levels of EGFR，MMP-2were detected by Real-time PCR and Western-blot.Results: The inhibitory rate and apoptosis rate of combination groupwere higher than cetuximab group, paclitaxel group（P＜0.05）; EGFR,MMP-2levels of combination group were lower than cetuximab group,paclitaxel group（P＜0.05）.Conclusion: The combination of Cetuximab and PTX can increase theapoptosis and decrease the proliferation of nasopharyngeal carcinoma cellline CNE-1with a synergistic action.The inhibition of EGFR, MMP-2was its possible molecular mechanism.