ATP Inhibition Of HBV Expression Study Compared With Lamivudine Role

Objective:An HBV X protein promoter targeted putative ZFP wasdesigned using Zinc finger tools for anti-Hepatitis B virus (HBV) therapy.The effect of the ZFP was evaluated in vitro in comparing with Lamivudine(3TC).Methods:1. Recombinant vectors ATF, ZFP1and ZFP2weregenerated by inserting the full or incomplete sequence encoding the ZFPinto the eukaryotic expression vector pcDNA3.1(+).2.Enzyme digestionwas performed to verify the structure of vectors3.Western blot and cellimmunofluorescence was used to evaluate the expression of ZFP inHepG2.2.15cells.4.In HepG2.2.15cells, at2days,6days and10days afterZFP transfect-ion or3TC treatment, western blot, Fluorescent quantitativePCR and ELISA was applied to evaluate the expression of HBx, thevolume of HBV DNA and the secretion of HBsAg and HBeAg,respectively.Results:1:The results showed that the recombinant plasmids wereconstructed correctly which could express ZFP well in the transformedHepG2.2.15cells;2: ATF and3TC reduced HBV X protein and HBV DNA significantly at all time points. The secretion of HBsAg and HBeAgin ATF and3TC group was also significantly decreased (P<0.05). Theeffect of ATF was stronger than of3TC(P<0.05). The effect at6days and10days after treatment was stronger than at2days (P<0.05).3: ZFP1andZFP2for X protein and without inhibition HBV DNA.Conclusion:The putative ZFP could be successfully expressed inHepG2.2.15cells and functioned as a HBV inhibitor which was moreeffective than Lamivudine with different mechanisms.