Hemin Offers Neuroprotection Through Inducing Exogenous Neuroglobin In Focal Cerebral Hypoxic-ischemia In Rats

Objective: To investigate the inducible effect of hemin on exogenousneuroglobin (Ngb) in focal cerebral hypoxic-ischemia in rats.Methods:125healthy SD rats were randomly divided into five groups:sham-operation control group, operation group, hemin treatment group,exogenous Ngb treatment group, and hemin and exogenous Ngb jointtreatment group. Twenty-four hours after focal cerebral hypoxic-ischemia,Ngb expression was evaluated by immunocytochemistry, RT-PCR, andwestern blot analysis, while the brain water content and infarct volumewere examined.Results: Immunocytochemistry, RT-PCR, and western blot analysesshowed more pronounced Ngb expression in the hemin and exogenous Ngbjoint operation group than in the hemin or exogenous Ngb individualtreatment groups, thus producing significant differences in brain watercontent and infarct volume [p <0.05].Conclusions: Hemin may be beneficial in protecting against focalcerebral hypoxic-ischemia through inducing the expression of exogenousNgb.