The Use Of Hyaluronatetoregulate Human Umbilical Vein Endothelial Cells Adhesion And Proliferationon Pcl/gelatin Composites

Tissue loss or organ failure due to severe disease or trauma is a major healthcareproblem, as the transplantation of the tissue or organ is not economical and limited by theaccessibility of compatible donor. Tissue engineering has been emerged as a promisingalternative strategy to treat patients with tissue loss or organ failure, which has the potential torevolutionize the treatment of many diseases. The design of scaffold is important for tissueengineering. Ideally scaffolds should have a proper structure similar to natural extracellularmatrices (ECM) which induce regenerative processed by interacting with relevant cellpopulations.Electrospinning provides a powerful means of fabricating scaffolds with structuresresembling those of native extracellular matrix. The properties of electrospun scaffolds arecontrolled by regulating electrospinning conditions, and their bio-affinities are improved byincorporating native extracellular matrix components. Electrospun nanofibers are preparedwith mixtures of polycaprolactone (PCL) and gelatin that can behave cooperatively todemonstrate combinations of structural and biochemical properties for tissue engineeringapplications. However, information on the bio-affinities of these scaffolds is lacking despitethe widespread use of PCL/gelatin scaffolds in biological studies and in the tissueregeneration field. In this study, we developed a composite nanofibrous scaffold composed ofhyaluronan (HA), gelatin, and PCL blends via one-step emulsion electrospinning. The meandiameter of the composite nanofibrous scaffold (as observed using scanning electronmicrographs) was approximately100-200nm; this dimension is similar to that of nativeprotein within the extracellular matrix.Attenuated total reflectance (ATR) FT-IR spectrarevealed that the resulting electrospun nanofibers contained both PCL, gelatin and HA. Also,addition of HA component transformed current PCL/gelatin component into moderatelyhydrophilic nanofibrous scaffold, which was the optimal cell adhesion surface. In vitrocultivation of Human Umbilical Vein Endothelial Cells (HUVEC-C) on the HA-based scaffolds showed a significant increase in cell adhesion, proliferation and filopodiaprotrusions. These findings suggest that such an HA-based nanofibrous scaffold might be apotential candidate for tissue engineering applications.