The Relationship Between Gut Bacteria And Inflammation Factors With Type2Diabetes Mellitus Of Uygurs And Kazaks In Xinjiang

Objective:Via analyzing the expression levels of gut Desulfovibrio, Clostridium bacteria, Lactobacillus, Akkermansia muciniphila, Bacteroides Thetaiotaomicron in Xinjiang Uygur, Kazaks people with normal glucose tolerance and type2diabetic population and the correlation between them and plasma lipopolysaccharide binding protein (LBP), soluble CD14(sCD14), tumor necrosis factor a(TNF-a), interleukin-10(IL-10), interleukin-1β (IL-1β), interleukin-6(IL-6), to investigate possible mechanisms of the impacts of intestinal flora on type2diabetes in Xinjiang Uygur and Kazaks. Methods:We analyzed the structural of intestinal bacteria by using Illumina second-generation sequencing methods and elected different gut flora in both Kazaks and Uygur with normal glucose tolerance and type2diabetes. We validate the different gut flora by method of Real-time quantitative (q-PCR). Detecting levels of LBP, sCD14, TNF-α, IL-10, IL-1β and IL-6in plasma sample of Kazakhs and Uygur by using enzyme-linked immunosorbent assay. Finally, Analyzing the differences in gut flora between groups using the statistical t-test, and analyzing the correlation between Clostridium, Desulfovibrio, Lactobacillus, Akkermansia muciniphila, Bacteroides multiforme and LBP, sCD14, TNF-α, IL-10, IL-1β, IL-6using Pearson analysis. Result:1. We found the differences gut flora (Clostridium family, Desulfovibrio family) by Illumina sequencing, and found possible differences gut flora (Lactobacillus, Akkermansia muciniphila, Bacteroides Thetaiotaomicron) in Xinjiang Uygur and Kazak by reading the literature.2. Real-time PCR results:①Compared with normal glucose tolerance group in Kazaks, the expression levels of genus Desulfovibrio is significantly increase in Kazaks with type2diabetes. Compared with type2diabetes in Uygur, the expression levels of genus Desulfovibrio is significantly increase in Kazaks with type2 diabetes.②Compared with type2diabetes in Uygur, the expression levels of clostridium is significantly increase in Kazaks with type2diabetes.③Compared with normal glucose tolerance group in Kazaks, the expression levels of genus Lactobacillus is significantly increase in Kazaks with type2diabetes. Compared with type2diabetes in Uygur, the expression levels of genus Lactobacillus is significantly increase in Kazakh with type2diabetes.④The abundance of Bacteroides Thetaiotaomicron in Uighur with T2DM was significantly higher than NGT group in Uighur.⑤The abundance of Akkermansia muciniphila was no significant differences among the groups.3. Elisa results:①The levels of plasma IL-1β in Kazakh with T2DM group was significantly higher than NGT group in Kazakh and in Uighur with T2DM group was significantly higher than the NGT group in Uighur.②The levels of plasma IL-6in Kazakh with T2DM group was significantly higher than NGT group in Kazakh.③The levels of IL-10in Uygur T2DM was significantly lower than NGT group in Uighur.④The levels of plasma TNF-a in Kazakh with T2DM group was significantly higher than NGT group in Kazakh and the levels of plasma TNF-a in Uighur with T2DM group was significantly higher than the NGT group in Uighur and the levels of the NGT group in Kazakh was significantly higher than the NGT in Uighur.⑤Compared with NGT in Kazaks, we found the levels of LBP and LBP/sCD14in T2DM groups in Kazaks had a statistically significant increased respectively, and also the levels of them in NGT of Uygurs had a statistically significant higher.4. The Pearson correlation analysis results between Inflammatory markers and some indicators:①There were significantly positively correlated between IL-6and FBG, TNF-α and FBG, IL-1β and FBG. There was significantly negatively correlated between IL-10and FBG.②There is a certain correlation between LBP and FBG, weight, BMI, TC, TG, HDL-C, or between LBP/sCD14and FBG, TG.5. The Pearson correlation analysis results between Flora and indexes:①There was significantly positively correlated between Desulfovibrio and FBG, Weight, BMI, waistline, and significantly negatively correlated between it and HDLC. There was significantly positively correlated between Desulfovibrio and IL-1β, IL-6, TNF-a, LBP, sCD14.②There was significantly negatively correlated between clostridium and Weight, BMI, and significantly positively correlated between it and HDLC. There was significantly positively correlated between clostridium and IL-1β, IL-6, IL-10.③There was significantly negatively correlated between Lactobacillus and Weight, BMI, waistline, and significantly positively correlated between it and HDLC, IL-6, TNF-a.④There was significantly positively correlated between Akkermansia muciniphila and Height, IL-10, respectively. There was significantly negatively correlated between Akkermansia muciniphila and BMI, CT, LBP, respectively.⑤There was a significantly positively correlation between Bacteroides thetaiotaomicron and IL-1β, TNF-a, respectively. Conclusion:1.The changes of expression about Desulfovibrio, Clostridium, Lactobacillus, Bacteroides thetaiotaomicron in Kazaks and Uygur with type2diabetes, suggesting that may be one reason that result in type2diabetes.2. There is a certain correlation between Desulfovibrio, Clostridium, Bacteroides Thetaiotaomicron, Akkermansia muciniphila and inflammatory cytokines, respectively, which can provide new ideas for research the pathogenesis of type2diabetes in Xinjiang Kazakh and Uygur.