Effects And Mechanism Of Low Frequency Stimulation Of Pedunculopontine Nucleus On Spontaneous Discharges Of Globus Pallidus Internus In Rats

ObjectiveBy observing the low-frequency electrical stimulation in Parkinson（PD）model rats on Pedunculopontine Nucleus (PPN) and the effects ofneurotransmitters (GPi) neurons discharge in the medial part of the globuspallidus, to explore the mechanism of the low-frequency electrical stimulationon pedunculopontine nucleus to treat the PDMethods30SD rats were randomly assigned to the control group and the PDmodel group in15. PD model was established by injection of6-OHDA in theRight SNc, seven glass microelectrode was used to observe and record lowfrequency electrical stimulation, micro-electrophoresis glutamate (Glu) and itsinhibitor MK-801, γ-aminobutyric acid (GABA) and its inhibitor bicuculline (BIC)and Microiontophoresis acetylcholine (Ach) and its inhibitor (ATR) of theaffective discharge frequency on rat GPi neuronalResultsGPi neuronal electrical stimulation frequency of PD rats was significantlyhigher than normal（P<0.05）；low frequency electrical stimulation of PPN, GPiwere all inhibited in control and PD rats, and the average dischargefrequencywas lower than normal（P<0.01); micro-electrophoresis BIC，Gluand ACh all played excitatory roles on neurons, while microiontophoresis ofMK-801and GABA neurons were inhibited. On the basis ofmicro-electrophoresis of BIC excitatory effects of low-frequency electrical stimulation can make the PPN neuronal firing frequency increased, and on thebasis of micro-electrophoresis MK-801and ATR on PPN low-frequencystimulation on inhibitory neuronal discharge frequency to further reduce.ConclusionLow frequency electrical stimulation inhibits GPi PPN neuronal activity,probably by regulating neurons projecting to the GPi Glu and GABA and Achnerve pathways to achieve，the result of the GABA pathway is more obvious.