The Influence Of Pagosid On NFP And CCL5in EAE Rats’ Spinal Cord

Objective:By observing the influence of Pagosid on neurofilament protein and ChemokineLigand5in EAE rats’ spinal cord, explore if Pagosid have neuroprotective effect onEAE, and its potential mechanism of action, to provide an new idea and experimentalbasic for MS clinical therapy.Methods:Divided78adult female Wistar rats into five groups randomly: normal group(N group), Immune induction group(EAE group), low-dose of Pagosid group (LPGSgroup), high-dose of Pagosid group (HPGS group) and prednisone acetate tabletsgroup (PAT group). Induce EAE model by using the self-made completely immuneantigen (GPSCH-CAF). No actions on N-group; do not give any medicine after theEAE group model built; give20mg/kg·d Pagosid to LPGS group while100mg/kg·dPagosid to HPGS group by intragastric administration; give8mg/kg·d prednisoneacetate tablets to PAT group by intragastric administration. Compare the morbidity ofeach group and record each group’s neurologic severity score. Execute those rats onthe7th,14th and21th day, and then to Bielschowsky silver staining andimmunohistochemical stain of NFP and CCL5on their spinal cord.Results:1. Morbidity of each group:88.89%of EAE group; the morbidity of PGS andPAT groups are lower than EAE group (P<0.05); the morbidity of HPGS groupis lower than LPGS group (P<0.05).2. Neurologic severity score: record the scores on the7th,14th and21th daysafter immunosuppression induction. On the14th and21th days, the neurologicseverity scores of LPGS, HPGS and PAT groups are lower than EAE group.On the14th day, the neurologic severity scores of HPGS and PAT group are lower than LPGS group (P<0.05). Compare the7th,14th and21th days’ group,they have statistical significance (P<0.05), but compare the7th and21thgroups are not statistically significant (P>0.05).3. Histopathology: after the Bielschowsky silver staining, the most of N-groupaxons are running directly, and close constructed, continuously and thicknessuniformity; while the rest of groups are not so good, some kind of axonchanged shown, such as, structural disorder, bad continuity and vacuolardeletion and broken.4. Immunohistochemical stain:4.1The expression of NFP on rat spinal cord: The result is that five groups areall positive expression of NFP, but the N-group is the most one. Thepositive expression of NFP on EAE group is obviously less than N-groupof the corresponding period, nevertheless, on HPGS, LPGS and PATgroups, the number of NFP positive neurons are more than EAE group ofthe corresponding period, and the difference has statistical significant(P<0.05). Compare the7th,14th and21th days’ group, they are statisticalsignificance (P<0.05), but compare the7th and21th groups are notstatistically significant (P>0.05).4.2The expression of CCL5on rat spinal cord: The result is that all groups areCCL5positive expression but N-group. And the CCL5positive expressionon EAE is obviously more than other groups of the corresponding period.The CCL5positive expression of medicine meddling group is a bit lower.Compare the CCL5positive expression of HPGS, PAT and LPGS groups,the LPGS group is lower than other two groups, the difference hasstatistical significant (P<0.05). Compare the7th,14th and21th days’group, they have statistical significance (P<0.05), but compare the7th and21th groups are not statistically significant (P>0.05).Conclusions:1. Pagosid can intervene and reduce the incidence of EAE model, meanwhile improve its axon damage, and its neuroprotective effect is associated with the dose.2. Pagosid have neuroprotective effect on EAE, it’s in relation to up-regulateNFP’s and down-regulate CCL5’s expression.