Sodium Selenite Improves Non-alcoholic Fatty Liver Disease By Reversing The Abnormal Thyroid Hormone Transformation

ObjectiveTo investigate the transformation situation of thyroid hormones in NAFLD andstudy the relationship between thyroid hormone and the disorder of lipidmetabolism. To research the effect of sodium selenite on non-alcoholic fatty liver byreversing abnormal transformation of thyroid hormone.Methods(1)64healthy male SD rats were randomly divided into model group and normalgroup, each for32. Rats in the model group were fed with high-fat diet for making ofnon-alcoholic fatty liver model and rats in the normal group were normally fed. Toobserve the change in body weight, liver weight and liver index at the time of0,4,8,12W, and detect serum AST, ALT, TG, TC, HDL-C, LDL-C, T3, T4, rT3,selenium content and liver5’-deiodinase activity of each group in different time point.HE and Masson staining were used to observe pathological changes in rat liver tissueby light microscopy.(2)96healthy male SD rats were randomly divided into normal group, modelgroup and selenite group, each for32.The normal group and model group rats werefed in the same way as described above.The selenite group rats were gavaged withsodium selenite (0.2mg/kg) after high-fat diets feeding for4weeks.The generalindicators, serum markers and pathological changes of each group were measured asdescribed above.Results1、(1) General indicators: The body weight, liver weight and liver index level ofmodel rats were significantly higher than normal group at12W (P<0.05).(2)Pathological examination: Hepatic tissue of model group rats showed obvioussteatosis, inflammatory cell infiltration and periportal fibrosis.(3) Serum indicators:At12W, the serum AST, ALT, TC, TG, LDL-C levels of model group significantly elevated. Comparing with the level in previous time points, the resultshad a statistically significant (P<0.05).(4) The level of thyroid hormone: T3and T4level of model rats decreased as the feeding time ncreased (P<0.05), while rT3levelincreased.The results showed a statistically significant compared with the normal gro-up at8,12W (P<0.05).(5) Liver5’-deiodinase activity and selenium content: The5’-deiodinase activity and selenium content level of model rats gradually decreased.Comparing with the normal group, the model group showed a statistically significant(P<0.05).2、(1) General indicators:The body weight, liver weight and liver index level ofsodium selenite group were less than the model group at12W.(2) Pathologicalexamination: Hepatic tissue fat deposition and fibrosis in sodium selenite group weresignificantly lower than model group.(3) Serum indicators:Serum AST, ALT, TC, TG,LDL-C levels of sodium selenite group after4W were lower than model group insame time point(P<0.05).(4) The level of thyroid hormone: T3and T4level of sodiumselenite group decreased, while rT3level increased.They were close to the normalgroup level at12W. There were no statistical differences between model group andsodium selenite group at12W (P<0.05).(5)Liver5’-deiodinase activity and seleniumcontent:5’-deiodinase activity and selenium content of sodium selenite group incre-ased gradually from4W. They were close to the normal group level at12W. Compa-ring with the model group, the sodium selenite group showed no statistical-ly significant at12W (P<0.05).Conclusion1、 The serum T3and T4lever decreased and rT3level increased significantly inNAFLD rats with high fat, which indicated that the level of thyroid hormone wasclosely related to NAFLD.2、 Liver selenium content and5’-deiodinase activity increased significantly inNon-alcoholic fatty liver disease rats, which was related to abnormal thyroidhormone transformation. 3、 Sodium selenite could increased liver selenium content and5′-deiodinase activityin NAFLD rats, and improved abnormal thyroid hormone transformati-on condition.The level of serum lipid disorder and liver lipid deposition an-d fibrosis appropriately improved.