Influence Of Transfectionn Of CX3CR1Gene On Migration Of Mouse Bone Marrow-derived Mesenchymal Stem Cells By Fractlkine In Vitro

ObjectiveTo isolate, culture and identify mouse bone marrow-derivedmesenchymal stem cells (MSCs); with the Transwell co-culture system toinvestigate the roles of Fractalkine in triggering the migration of the MSCs.MethodsMSCs were isolated,culture through the whole bone marrow method,amplified by the different-speed adherent method. The morphology andbiological characteristics of MSCs was examined by flow cytometry. Andthen the recombinant adenovirus containing CX3CR1and greenfluorescent protein (GFP)（LV-CX3CR1-EGFP）or empty vector （LV-CON-EGFP）was transfected into MSCs. The expression of CX3CR1mRNA in MSCs was examined by RT-PCR and the protein expression wasdetected using Western blotting in LV-CX3CR1-EGFP infection group,LV-CON-EGFP infection group and uninfection group.The migration ofMSCs was detected with Transwell co-culture system. ResultsObtained MSCs were homogeneous in cell mophous with good livingcondition and the cells showed positive with CD29、CD44、CD34.Therewas no expression of CX3CR1in LV-CON-EGFP group and uninfectiongroup but in the LV-CX3CR1-EGFP group we have investigated that theCX33CR1can be expressed.The result of Transwell assays suggested thatthe migration levels of MCSs was higher in LV-CX3CR1-EGFP group thanother groups（P<0.05,P=0.000）.ConclusionThe transfection of CX3CR1gene could improve the level of migrationof MSCs by fractlkine in vitro.