| Doxorubicin Hydrochloride is a broad spectrum antineoplastic agents,has a strong killing effect on tumor cell. But also easily lead to cardiactoxicity and bone marrow suppression and other side effects. Thetraditional methods have high-performance liquid chromatography,spectrometry, radioimmunoassay and laser-induced fluorescence method.These methods are low sensitivity or complicated operation,time-consuming. Therefore, the rapid determination for DoxorubicinHydrochloride of high-sensitivity has important clinical value formonitoring drug plasma levels of cancer patients. Flow injection analysis isa modern quantitative technology which the liquid samples areautomatically processed and detected online in the non-equilibrium state.Compared with the method of radioimmunoassay and enzyme-linkedimmunosorbent analysis, chemiluminescence method has many advantages,such as high sensitivity, good stability and wide linear dynamic range.Therefore, flow injection chemiluminescence is important trace analyticaltechniques in the field of organics, inorganics and drug analysis, and has been widely used in environmental monitoring, determination of drug,clinical biochemistry, chemical reaction kinetics research and industrialanalysis online.This paper studies the effect of Doxorubicin Hydrochloride onluminescence system of luminol and ferricyanide, established a highsensitivity for the rapid detection method of Doxorubicin Hydrochloride. Inthe optimal experimental environment, the linear detection range was0.4~2.5μg/mL; the detection limit was2.9×10-2μg/mL. The relativestandard deviation of2.1%was obtained for consecutive determinations of1.2μg/mL Doxorubicin Hydrochloride，and the recoveries were in therange of98.4%~102.0%. As the public health is concerned by most people nowadays, thequalitative and quantitative analysis of bacteria at the earliest stages ofinfectious diseases is the key to prevent major public health events.Salmonella typhimurium (S.Typhimurium) is considered as one of the mostcommon foodborne pathogens which caused significant infections ofmorbid and mortal world widely. Actually a thimbleful S. Typhimuriumcells can cause serious typhoid fever and even death. However, thetraditional analysis methods included specific enrichment media to culture,distinguish, and count bacterial colonies takes at least two days after thesample is obtained. The polymerase chain reaction (PCR) detectionmethods require pretrea tment steps to condition the biological samples andto extract the suitable target DNA. To overcome these drawbacks, there hasbeen a continuing search, such as surface plasmon resonance (SPR),electrochemical immunosensor, magnetoelastic biosensors, quartz crystalmicrobalance (QCM) and potentiometric sensor. But these methods aredifficult to quantify the ultralow concentration of bacteria accurately.Therefore, the development of an ultrasensitive, specific, culture-independent analytical approach for quantifying viable pathogenicmicro-organisms cells detection is necessary.Aptamer as a recognition element has gained more and more attentionand research in the field of electrochemical biosensing. It is able to identifythe specific target substances, which will help people to build a variety ofsensor types, has a profound impact on the development of analysischemistry. In order to improve the measurement sensitivity of the assaytarget substance, amplification of weak signals is an important solution.Among them, the use of nano-materials and new signal amplificationtechnology is an important part of the program.Hence we present an ultrasensitive electrochemical biosensor based ontarget-induced aptamer displacement and cascade signal amplificationtechnique for the specific quantitative detection of the livingS.Typhimurium. The fabricated aptasensor could provide both a wide lineardynamic range (2×102~2×108cfu mL-1) and a low detection limit (38cfumL-1), and has good reproducibility and specificity. It is critical for a sensorto achieve sensitive detection in real samples. In the real samples, the DPVelectrical signal response from2×102to2×108cfu mL-1with a a limit ofdetection of102cfu mL-1. |
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