Experiment Research Of Hypoxia Inducible Factor-1α^mu Transfected Into EPCs To Effect The Expression Of CXCL12and The Migration Of EPCs

ObjectiveTo observe the expression of CXCL12from the EPCs transfected byAdv-HIF-1αmuand investigate the effect to the EPCs.MethodsUnion inducting and culturing the MNCs separated by density gradientcentrifugation combined with VEGF、EGF and bFGF into EPCs and identifyingits angiogenic activity; Adv-HIF-1αmu transfected into EPCs by the optimummultiplicity of infection （MOI）,and the EPCs containing the purpose gene asgroup A obtained. The EPCs transfected merely Adv as group B.The EPCs nottransfected Adv-HIF-1αmu as group C.The expression of HIF-1in transfectedEPCs were determined by Western Blot, the concentration of supernatant byELSA and the migration of exogenous EPCs by transwell.Results1、The MNCs obtained by density gradient centrifugation can be inducedand cultured into EPCs by EGF、bFGF and VEGF，which can combine withUEA-1and uptake ac-LDL and are angiogenic;2、Group A (i.e. the transfection Adv-HIF-1αmu) of EPCs can expressHIF-1, and group B (not transfection Adv-HIF-1αmu EPCs) and group C (puretransfection Adv the EPCs) no expression of HIF–1；3、 ELISA showed that expression of CXCL12is up-ragulated intransfected EPCs compared with other two groups, there is no obvious differences between group A and B；4、The supernatant fluid of EPCs after transfection can promote themigration of exogenous EPCs, the migration quantity of cells in group A ishigher than in group B and group C (P>0.05), no obvious difference wasfound between group B and group C (P <0.05)ConclusionsThe MNCs obtained by density gradient centrifugation can be induced andcultured into the characteristic EPCs within EGF、bFGF and VEGF，and theEPCs transfected by Adv-HIF-1αmucan express highly HIF-1and CXCL12，and induce the migration of exogenous EPCs.