Preparation And Characterization Of Calcium Phosphates/Gelatin Porous Scaffolds

Nowadays, Porous scaffolds with good biocompatibility and bioresorbability became the focus of researches on tissue engineering. Preparation and characterization of calcium phosphates/gelatin porous scaffolds were investigated in this paper.Firstly, P-TCP/HA biphasic composites were prepared by chemical precipitation method. The effects of pH value, initial Ca/P molar ratio and calcining temperature on the composition and crystal phase of synthesized powders were investigated. The method was developed to eliminate a-TCP phase in TCP/HA composites, which was not found in other reports.Secondly, the transformation of HA precursor (initial Ca/P molar ratio is1.667, amorphous) and TCP precursor(initial Ca/P molar ratio is1.50, amorphous) was studied. Compared with conventional chemical precipitation method, the precursor conversion method was proposed in order to prepare calcium phosphates for the first time. It was found that the composition of the products can be controlled to some extent, and a-TCP/HA biphasic composites were synthesized successfully.At last, calcium phosphates/gelatin porous scaffolds were prepared with freeze-drying method. Range of the pore sizes is from200μm to500μm. The in vitro degradation behaviors of different calcium phosphates/gelatin scaffolds in simulated body fluid (SBF) were detected. Among them, HA/gelatin scaffold showed the lowest degradation rate, while a-TCP/HA/gelatin scaffold showed the highest. The antibacterial activity of calcium phosphates/gelatin/cerous nitrate porous scaffolds was investigated using the thick flat cave method. The results showed that the antibacterial activity of scaffold against Staphylococcus Aureus was outstanding with certain amount of cerous nitrate.