Herpes Simplex Virus Type â…  Ul43Gene Expression And Preliminary Study For The Function

Objective:According to the bioinformatics analysis, a preliminary study on the function by construtingHSV-1UL43eukaryotic expression vector, and Providing a reliable experimental basis for furtherstudy and for on the new antivirus medicine.Method:1. Studying HSV-1UL43genetic evolution, protein structure and function by a largenumber of bioinformatics software, such as DNA star, Protpara,http://bp.nuap.nagoya-u.ac.jp/sosui/, http://mendel.imp.ac.at/sat/DAS/DAS.html and soon.2. According to the genetic engineering principle, construting the HSV-1UL43T vectorand eukaryotic expression vector.3. Construting the HSV-1four glycoproteins (gB, gD, gH-gL) T vectors and eukaryoticexpression vectors, and transient transfection for CELISA. To study fusion moreefficiently and effectively, we have employed a virus-free cell–cell fusion assay.Results:1. TheUL43gene and gene products are conserved only within the Alpha-andGammaherpesvirinae subfamilies. The homologue of CeHVUL43was the closest, butthe homologue of Prv UL43was higher than the homologue HHV-3UL43andHHV-8UL43.2. The HSV-1UL43p contained many phosphorylation and glycosylation sites. The numberof transmembrane predicted was8.3. These genes were cloned and sequene. The UL43protein of HSV-1has been identifiedby epitope-tagging as a34-kDa polypeptide.4. The UL43p that were expressed on the effector cell surface could moderate reducesHSV-induced cell-cell fusion in dose-dependent.Conclusion: As a transmembrane protein, the UL43p like UL20p is not the four necessary glycoproteinsrequired for virus entry to induce cell-cell fusion. But UL43can mediate the negative control offusion and reduce HSV-induced cell-cell fusion.