Biofabrication Of Bacterial Cellulose And PLGA Via Layer-by-layer Rolling-based Fabrication Technique For Tissue-engineered Blood Vessels

Cardiovascular diseases are the leading cause of premature death worldwide. The limitedavailability of healthy autologous vessels for surgical bypass grafting procedures has ledto the fabrication of artificial vascular scaffolds. While numerous polymers have beenextensively studied as substitute in vascular engineering, they fall short of meeting thebiological challenges at the blood-material interface. Various tissue engineering strategieshave emerged to address these flaws and increase long term patency of vascular grafts.Bacterial cellulose (BC) and poly (lactic-co-glycolic acid) PLGA have been widely studiesfor many tissues and organs engineering for their unique properties. In this study, we triedto give full inside into mechanical properties of tubular BC and PLGA fabricated by usinglayer-by-layer manual rolling-based fabrication technique for tissue-engineered bloodvessels. We evaluated biocompatibility and haemocompatibility of BC and PLGA. Ourresults demonstrated that neither BC nor PLGA elicit any foreign body response. Afterbeen incubated in the PRP for30mins, BC elicit no platelet adhesion which ensure lessthrombogenicity compare with PLGA which show low level of platelet adhesion but noneactivated. BC was found to hold great potential of mimicking native blood vessel as itexhibited desirable mechanical properties in comparison with PLGA which showed weakmechanical strength in contrast to BC. The fabrication technique that we have employedhere holds an enormous prospective of fabrication any kind of tubular shape with precisecontrol feature for any particular uses in tissue engineering.