| Purpose:Dynamic observation to medical reinforcement technique for the treatment ofadult male rabbit model of free fall contusion effect, The organization principle andexperimental technology such as chemistry, molecular biology, in the histologic assessment,IGF1and IGF1mrna and the damage indexes of myocyte apoptosis rate, exploring backmedicine with reinforcement technique of mechanical stimulation in the process of for thetreatment of acute soft tissue injury, whether its biological effect mechanism is throughstimulation of mechanical stimulation growth factor (IGF1, enhanced endogenous mrnaexpression of IGF-1, a large number of IGF1is activated, accelerate the process ofmyoblast proliferation, thus promote the possible mechanisms of injury tissue repair.Methods:174New Zea land big-eared white rabbit experimental rabbit random number,1-24set as blank control group, only do not do any processing, compare in eight points ineach executed three observations;The rest of the25-174for dynamic observationgroup.Dynamic observation group150rabbit by using the method of2.0Kg weight free-fallcontusion posterior limb of gastrocnemius, building after building were randomly dividedinto5groups.Group1to group B:40, only1day after building began to go back to medicalreinforcement technique treatment,1times a day, a total of20times, and dynamicobservation points and eight executed five rabbit based observations;2groups were set to C:35, only2days after the building back to reinforcement technique treatment, medicine, oncea day, a total of19times, dynamic observation points in the subsequent observation of7eachexecuted five rabbit based observations;3groups were set as D:30, in3days after thebuilding began to go back to medical reinforcement technique treatment, once a day, a total of,18times, in the follow-up of6dynamic observation points each executed five rabbit basedobservations;4groups were set to E:25, only4days after building started back toreinforcement technique to treat medicine, once a day, in total,17times, in the follow-up of5dynamic observation points each executed5rabbit based observations;Group set to F5:20, only5days after building started back to reinforcement technique treatment, medicine, once aday, a total of16times, in the follow-up of45rabbit based dynamic observation points eachexecuted observation.And by immunohistochemical method, the WB (Western blot) method,real-time fluorescent quantitative PCR method and image analysis system to observe the bigwhite rabbit after calf injury in the process of natural restoration and The hui nationalitymedicine massage technique the expression of IGF1after treatment and recovery.Results:(1) after acute contusion natural recovery group of IGF1and IGF-1mRANrelatively integral optical density value compared with blank control group, control groupexpression of skeletal muscle did not see;1day after the injury that has a weaker expression,then express the amount gradually rise, peak, five days later gradually decline, there are stillexpress20days.(2)1-5days after injury, compared with natural recovery group, the techniqueof intervention group the dynamic observation points of IGF1and IGF-1mRAN expressionall have significant difference, the overall trend is higher;5days after natural recoverytechnique group and intervention group of IGF1and IGF-1mRAN expression gradually;1-4days after the injury early intervention group difference is not big, the fifth day of interventiongroup reasonable reinforcement technique after the intervention of IGF1and IGF-1mRANexpression significantly enhanced, weakened the late recovery is relatively obvious, the otherintervention group had significant difference.(3) natural recovery and back to reinforcementtechnique medicine intervention group A B-group F the first5days after injury, each tissuedamage fiber cell apoptosis positive expression of trends to increase gradually, and fade outafter5days;Apoptosis of B-group E, F group5days apoptosis significantly enhanced afterintervention, less evident, after20days close to normal, and other groups have significantdifference.Conclusion:(1) rabbit skeletal muscle moderately severe contusions natural recovery process change law is: after acute injury of natural repair starts2days after injury,5days toreach the best state of repair, after returning, to20days were not completely restored.(2) Thehui nationality medicine massage technique promote skeletal muscle damage repairmechanism is by regulating the expression of IGF1and IGF-1mRAN, damage in localregulation of cell proliferation and differentiation, effectively induced damage tissue cellapoptosis acceleration, accelerated by the fibroblasts of the muscle fiber cell transformation,and accelerate the tissue repair rate, finally achieve the purpose of healing. |
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