Role Of P38Signaling Pathway Of SCL-1Cells In Response To5-aminolevulinic Acids-based Photodynamic Therapy

Objective To explore the effect of P38signaling pathway in killing skinsquamous cancer cells （SCL-1）induced by5-aminolevulinic Acids-based(ALA)Photodynamic Therapy(PDT). The P38pathways may become a new therapeutic targetto enhance the curative effect of ALA-PDT on killing SCL-1cells, and providenew theory basis for the clinical in treating skin squamous carcinoma.Methods SCL-1cells were divided into control group、ALA group、light group、ALA-PDT group and inhibitor of P38group according to the different treatmentand have or not inhibitor. Western blot technique was used to detect theexpression of phospho-P38(p-P38)、 phospho-Elk-1(p-Elk-1) and PARP(polyADP-ribose polymerase) after intervening30min、60min、90min of each cell groups.MTT was used to calculate the cell survival rate after optical density valueof each group were obtained at24h、48h、and72h. Flow cytometry with AnnexinV-FITC/PI double staining technique was employed to detect apoptosis of eachcell after intervening24h、48h and72h.Result The expression of p-P38and p-Elk-1were significantly higher thanthe other groups, especially at the60min and90min point. PARP of85KDa wasincreased significantly in inhibitor of P38group. Cell survival rate of ALA-PDTgroup and inhibitor of P38group were decreased significantly. Compared withcontrol group, ALA group and light group, the apoptosis rate of ALA-PDT groupwas increased remarkable, inhibitor of P38group decreased slightly than ALA-PDTgroup, but also higher than the other groups. Conclusion The activation of P38signaling pathway can inhabit cellapoptosis caused by ALA-PDT. Blocking this pathway may become a new therapeutictarget to enhance the curative effect of ALA PDT on killing SCL-1cells.