Methylation Analysis Of CHFR And FHIT Gene Promoter Region In Colorectal Carcinoma

Background and ObjectiveColorectal cancer is one of the most common malignant tumors in the digestive tract tumor and its morbidity stays third among malignancies in the world, its mortality accounts 9% of all tumors, and it always develops to middle-late stage when it is diagnosed, and it even has the poor therapeutic effect and prognosis. Therefore, it is important to explore the occurrence of molecular level, the development mechanism, and find the method of early diagnosis and treatment. In recent years, people realize that the formation of epigenetic modifications also involves in tumor. The DNA’s methylation was one of the earliest gene epigenetic modifications, abnormal methylation of genes may lead to inactivation, it has been found that the abnormal hypermethylation of multiple genes have frequent occurrence in colorectal cancer. CHFR(Checkpoint with FHAand Ring Finger), a divisionof normal cells to mitotic phase detection of Seolniek and Halazonetis in 2000, the new discovery and control division of the gene is a newly discovery, and tumor suppressor gene was the first detection point to be found in the prophase of mitosis. CHFR is widely expressed in normal tissues, and its structure and function are very conservative. Research shows that, the loss of CHFR expression in liver cancer, leukemia, stomach, breast cancer and so on many kinds of tumor tissues, the methylation of Cp G Island can lead to the loss of CHFR expression or silencing, but the research in colorectal cancer is seldom reported. A brittle group of amino acids(Fragi Ie Histidine Triad, triple FHIT) gene is the first tumor suppressor genes of fragile sites and tumor associated candidate. The study found that: " as a tumor suppressor gene, the FHIT gene the promoter methylation status is in high stage in a variety of tumors." In this study, using the methylation status of FHIT and CHFR methylation specific PCR was detected in 46 cases of colorectal carcinoma and corresponding adjacent normal tissues of genes, and its objective is to explore the role of colorectal carcinogenesis. Method1. The experiment uses methylation specific polymerase chain reaction(MSP)method for detecting, each of colorectal carcinoma and normal colorectal tissues has 46 cases, the main detection of CHFR gene and FHIT gene promoter methylation level sub area, and analyses the relationship between the two gene methylation level with age, gender, tumor location, differentiation degree, lymph nodemetastasis, clinicalstage andthe correlation between CHFR gene and FHIT gene methylation level.2.Statistical processing uses SPSS 17 statistical software to analyze relevant data, statistical methods were applied for analysis and Spearman correlation P< 0.05 is the difference with statistical significance. Result1.The methylation rate of CHFR gene tissue in colorectal cancer tissue and para-cancerous respectively is 49.15%, 8.69% with statistical significance difference(x 2=16.24, P< 0.05); the level of methylation and the degree of differentiation, lymph node metastasis(P< 0.05), and with the gender, age, tumor location and clinical stage significant correlation(P> 0.05).2.Methylation of FHIT gene in colorectal carcinoma and normal colorectal tissues respectively is 54.35%, 13.04%, the difference was statistically significant(2= 22.21, P<0.05); the level of methylation and the degree of differentiation, lymph node metastasis(P< 0.05), and with the gender, age, tumor location and clinical there was no significant correlation between staging(P>0.05).3.Statistical analysis shows that colorectal cancer CHFR and methylation of FHIT gene in the occurrence rate was positively correlated(r=0.393, P<0.05). Conclusion1.CHFR, FHIT gene’s methylation rate is significantly higher than that in para-cancerous tissues of 1 colorectal carcinoma metastasis, and significantly correlated with the degree of differentiation, lymph node, CHFR, showed that themethylation of FHIT gene may be involved in colorectal cancer development and metastasis.2.CHFR and FHIT gene’s methylation level in colorectal cancer related gene suggests that the two may be in the colorectal cancer play a synergistic role in the development, so the combined detection of CHFR and FHIT gene methylation level for diagnosis and prognosis of colorectal carcinoma has important value.