Human Apo M Down-regulates Expression Of Cell Death-inducing DNA Fragmentation Factor45-like C And The Study Of Its Mechanism

Atherosclerosis(Atherosclerosis, AS) is a threat to human health of common disease of heart head blood-vessel, among the global death factors in the second place,and its pathogenesis has not been fully elucidated, lipid metabolism disorder is the important reason for it to happen.Liver cell lipid metabolic disorder that excess triglycerides(Triglyceride, TG) gathered in cells, lead to high blood triglycerides, and causing atherosclerosis.Human apolipoprotein M(apolipoprotein M, apo M) is one of the newly discovered apolipoprotein, primarily in the liver cells and renal tubular epithelial cells express, involved in the formation of the former beta HDL and cholesterol reverse transportation and resistance to the AS, and the Peroxisome Proliferator- Activated Receptor Gamma(PPARγ) expression.PPARγ can be combined in Fat Specific Protein 27(FSP27) promoter region, and then adjust the expression of FSP27 in mouse cells.Cell death-inducing DNA fragmentation factor 45-like c(cidec)also known as FSP27, first isolated in the Fat cells in mice, then in human liver cells,Fat cells and other cells have been found that have close relationship with atherosclerosis, the expression mechanism of Cidec is not clear so far.The purpose of this study is to explore PPAR gamma adjust the molecular mechanisms of gene expression of cidec in human cells, for the treatment of diseases such as atherosclerosis provide new targets.On the basis of front research,first separation and purification person apo M protein, detected the regulation of the expression of PPAR gamma, and construct report gene plasmid about cidec promoter regions segments of different length,then for the report gene experiments to explore the molecular mechanism of PPAR gamma adjust the expression of cidec by transfection of Hep G2 cells.The experimental results are as follows:⑴Ion exchange chromatography is more efficient than ultracentrifugation on purification human apo M;⑵Human apo M can down regulation of PPAR gamma expression in human hepatocellular carcinoma cells;⑶ PPARγ could combine to human cidec promoter region- 2289-- 1800 bp areaand regulating the expression.