HME Inhibiting Angiogenesis And Its Role In Gastric Cancer

Objective To investigate the expression of HME, AS and MCP-1 in gastric cancer and para-carcinoma tissue,and the clear relationship between HME and AS and MCP-1 in human gastric carcinoma.Combined with the complete clinical follow-up data of patients,to explore the clinical significance. To generate eukaryotic expression vector of PEGFP-Cl- HME for further explore the mechanism of HME inhibition of gastric cancer.Methods The expression of HME,AS and MCP-1 in 30 specimens of gastric carcinoma and 30 specimens of normal gastric tissues were detected by immunohistochemical SP method.Combined with the complete clinical follow-up data of patients, the correlation of HME,AS and MCP-1 expression and its relationship with clinical pathology. were analysed. Extraction of RNA from human gastric carcinoma,c DNA fragment by RT-PCR amplification of HME gene encoding domain I and II, after the product was purified and cloned into PGEM-T vector, restriction analysis and DNA sequence by restriction enzyme analysis and identification after the gene fragment was cloned into the eukaryotic expression vector of p EGFP-C1 and double enzyme digestion and PCR identification.Results In gastric carcinoma,the percentage of HME,AS and MCP-1 positive expression was 66.7%(20/30),73.3%(22/30) and 80.0%(24/30) respectively. While in normal astric tissues was 10.0%(3/30),16.7%(5/30)and 16.7%(5/30) respectively. The expression in gastric carcinoma was significantly higher than that in normal gastric tissues, and the difference had a statistical significance (P<0.05).The expression of HME was significantly associated with the depth of the blood vessels invasion and degree of differentiation, nothing to do with other clinicpathological parameters. The expression of AS was significantly associated with the vascular invasion, tumor size, invasion, lymph node and distant metastasis, nothing to do with other clini-copathological parameters.The expression of MCP-1 was significantly associated with the depth of invasion, tumor size, tumor stage and lymph node and distant metastasis, nothing to do with other clinic pathological parameters.The expression of HME was positively correlated to that of AS in gastric carcinoma(Contingency analysis r = 0.570, P <0.01).The expression of HME was not correlated to that of MCP-1 in gastric carcinoma(Contingency analysis r = 0.115,P>0.01).HME genes have been cloned by c DNA eukaryotic cell expression vector p EGFP-cl plasmid with Bam HI and Kpn I double enzyme to produce two size stripe around 6.1KB and 1.4KB, consistent with the expected, p EGFP-C1-HME sequencing results showed that the nucleotide sequence HMEc DNA sequence of bases in line with with other people.Conclusion The expression of HME, AS and MCP-1 in gastric carcinoma was significantly higher than that in normal gastric tissues.Their expressions of varying degrees were closely related with the occurrence development,infiltration and transfer of gastric cancer.This may be a method of early diagnosis,prognostic judgment of gastric cancer and molecular target therapy for gastric cancer. The eukaryotic expression vector p EGFP-C1-HME was successfully constructed, the next step was transfected into gastric cancer cells, to further explore the pathogenesis of gastric cancer.