The Preliminary Study On Structure And Biological Activity Of Polysaccharide Fractions From Inonotus Obliquus

Inonotus obliquus(I. obliquus) belonging to the family of Hymenochaetaceae has been used as an effective folk medicine to treat a wide range of diseases such as cancers, gastritis, ulcers, and tuberculosis of the bones since the 16 th century. The preliminary structure and activities of the I. obliquus extracts were evaluated. These findings implied that the extracts have good antiproliferative, antioxidant and immunity activities, and further provided insights into the understanding of molecular basis for diverse medicinal values of this mushroom.The most important results of the thesis are listed below.1. Through the hot water-extraction or alkali-extraction procedure two crude polysaccharides(IOW and IOE) were obtained. Further purified by the ion exchange resin and gel column chromatography separation and purification, three compounds IOW-S-1, IOW-S-2, IOE-W1 with molecular weight of 37.9 kDa, 24.5 kDa and 61.15 kDa, respectively.2. Chemical and spectral analysis showed that both IOW-S-1 and IOW-S-2 were lignin-carbohydrate complexes.3. This study evaluate the antiproliferative activities as well as the relevant mechanism involved of IOW-S-1, IOW-S-2. The results have shown that both of them have good antiproliferative activity in a concentration dependent manner. Its mechanisms which tested by flow cytometry has exhibited the apoptosis induction was largely cell-cycle independent. Further investigation used immunofluorescence demonstrated that IOW-S-1 or IOW-S-2 inhibited the activation of the nuclear transcription factor in cancer cells.4. This study also tested the antioxidant and immune activity of IOE-W1, the result indicated it is a good antioxidant agent, could rescue H2O2-induced damage from H2O2-treated PC-12 cells, and also it is a very good immune activation material, it could promote NO production in RAW264.7 cells in a concentration dependent manner.