Anti-fatigue Effect Of Total Flavonoids From Lycium Barbarum Leaves In Mice

The aim of present study is to investigate the anti-fatigue effect of total flavonoids from Lycium barbarum leaves(TFL). Mice were randomly divided into five groups (motionless, swimming, high-dose, medial-dose and low-dose groups). The motionless and swimming groups were gavaged with distilled water, the other three groups were gavaged with TFL of 200, 100 and 50 mg/kg body weight, respectively. After 30 days, the swimming and medicated groups were performed a exhaustive swimming test and the exhaustive swimming time, tissue glycogen, blood lactic acid(LD), blood urea nitrogen (BUN), SOD activity and MDA content of liver, skeletal muscle and cardiac muscle, were examined. The ultrastructure of skeletal and cardiac muscle cells were observed by transmission electron microscope(TEM) and the expression quantity of SOD1 were observed by immunohistochemistry(IHC). The results showed that TFL could extend the exhaustive swimming time of mice, effectively increasing the tissue glycogen content and the SOD activity of liver, skeletal and cardiac muscles, and also effectively increasing the expression quantity of SOD 1, while decreasing the blood lactic acid, blood urea nitrogen contents and MDA content of liver, skeletal and cardiac muscles. TEM results showed that skeletal and cardiac muscle cells of swimming group were severely damaged, while the damage degree of high-dose group was obviously alleviated, approximating to the motionless group. All the results indicated that TFL had prominent anti-fatigue activity and could elevate the exercise tolerance.