Effect Of HPDLSCs-conditioned Medium On Cell Proliferation And Migration Of Tongue Carcinoma Cell Line Tca-8113

Objective:To investigate the effect of human periodontal ligament stem cells (hPDLSCs)-conditioned medium (CM) on cell proliferation and migration of tongue carcinoma cell line Tca-8113.Methods:hPDLSCs were isolated and culture-expanded from healthy adults. When the passage 3 reached 80% confluence, the medium was changed to fresh medium without fetal bovine serum. After 24 h incubation, the supernatant (hPDLSCs-CM) was collected. Then the Tca-8113 cells were incubated in fresh medium alone (as the control group) or in the medium supplemented with 50% hPDLSCs-CM (as the CM group). The cell proliferative activity was measured by MTT assay, and the cell cycle was analyzed by flow cytometry (FCM), and the cell migration ability was measured by transwell chamber assay.Results:hPDLSCs which were isolated and culture-expanded expressed mesenchymal stem cell-related antigen Stro-1 and CD 146, and showed the ability of colony-formation and multilineage differentiation. After Tca-8113 cells were co-cultrued with the hPDLSCs-CM for 3,5 and 7 d, the cell proliferation of the CM group were significantly higher than that of the control group (P< 0.01). After being co-cultured for 4 d, the cell proliferation index (PI) of the CM group was significantly higher than that of the control group (P< 0.01). After being co-cultured for 24 h, the cells passed across the membrane in the CM group was significantly more than those of the control group (P< 0.01).Conclusion:hPDLSCs-CM can promote the proliferation and improve the migration ability of Tca-8113 cells.