The Research On The Relevance Among TSH, Serum Resistin,URIC,and Homocysteine In Subclinicalthyroid Dysfunction

Objective:Subclinical thyroid dysfunction includes subclinical hyperth- yroidism and subclinical hypothyroidism. The level of free amino acid(FT3) and free thyroxine(FT4) levels were normal and the level of thyroid stimulating hormone(TSH)was abnormal in the serum of patients with subclinical thyroid dysfunction. Though the clinic symptom was mild,it was attracted much attention in recent years because of its potential pathogenic tendency and easily evolving into clinical thyroid dysfunction without treatment. Many complications such as hypertension, atherosclerosis and so on will be produced.linical thyroid dysfunction is a branch of advanced preventive medicine. The research of subclinical thyroid dysfunction will be helpful for disease prevention in advance.Resistin is newly discovered as adipocyte factor secreted by the adipocyte. Its clinical effect was gradually be found. Resistin played an important role in inflammatory response of diseases such as atherosclerosis, diabetes,brain damage,etc.At present,there are less for researches of the relationship between resistin and thyroid disease. the thyroid dysfunction and fewer on the relevance between resistin and subclinical thyroid dysfun- ction.The purpose of this study is to explore its relevance between the level of resistin and subclinical thyroid dysfunction.hyperuricemia is a disease in which the purine metabolic or uric acid excretion disorder.It was a risk factor of hypertension, atherosclerosis and proteinuria etc. Also cardiovascular disease and the important performance of insulin resistance.The research on the correlation between the subclinical thyroid dysfunction and serum uric acid was few in the domestic and interna- tional study.The purpose of this study is to analysis the the change of the serum uric acid and discuss its clinical significance in the patients with subcl- inical thyroid dysfunction.Homocysteine(Homocysteine,HCY) plays more and more important role in the pathophysiology and prognosis of cardiovascular disease(CVD). It was attacted attention because of promoting the subclinical hypothyroidism and increasing atherosclerosis.There are few research on the changes in the disease of subclinical hyperthyroidism.The purpose of this thesis was to analysis the correlation of plasma homocysteine and thyroid function. It will provide a feasible monitoring indicators for the patients with subclinical thyroid dys- function.Many complications such as hyperlipidemia, atherosclerosis and so on would be induced without effective treatment during the period of subclinical thyroid dysfunction, so it was particularly important to analyse its associated indicators. This study intends to detect serum Resistin, URIC, HCY levels of patients with subclinical thyroid dysfunction and the healthy control. The level changes of Resistin,URIC,HCY were found.This study was to provide exper- imental basis for preventing the incidence of complications of subclinical thyroid dysfunction.Methods:1 Research objectSeventy patients with subclinical thyroid diseases without treatment were randomly selected from July 2013 to July 2014.Among them 32 cases with subclinical hyperthyroidism were selected in the group of subclinical hypert- hyroidism and 38 cases with subclinical hypothyroidism were selected in the group of subclinical hypothyroidism. In the same period,72 cases of healthy volunteers were selected as the control group.TSH,Vit B12 and Folate were detected by the method of Luminescence Immunoassay.The serum resistin was detected through the method of ELISA,and homocysteine was detected on the method of enzyme. With the finish rate method determination of uric acid. To study the change of the serum resistin, URIC and homocysteine in serum of patients with subclinical thyroid dysfunction.2 Test method(1) The results of FT3,FT4,TSH, Vit B12 and Folate were detected on the method of Luminescence Immunoassay.All the test were in the secondary biological safety laboratory.(2) The levels of serum URIC,HCY and the conventional biochemical indexes:TC, TG, HDL- C, LDL- C were detected by automatic biochemistry analyzer in the secondary biological safety laboratory for testing.(3) The level of serum Resistin was detected: enzyme-linked immune method, according to the reagent instructions. All the test were in the secondary biological safety laboratory.SPSS16.0 statistical software is used to deal with all data. the data adopt mean±standard deviation,variance analysis comparing the selection among three groups,Spearman correlation analysis between the two indexes by corre- lation, All tests were two-sided, P<0.05 had statistical.Results:1 Three groups of general data comparison: subclinical hyperthyroidism group Subclinical hypothyroidism group and control group in age, gender, BMI,FT3 and FT4 had no statistical significance2 CHOL, LDL level in the group of subclinical hyperthyroidism was significantly lower than control group(P<0.05), lower than that of subclinical hypothyroidism group(P<0.05); Patients serum TG CHOL, subclinical hypothyroidism group was obviously higher than that of control group, significantly higher than that of subclinical hyperthyroidism group, the difference was statistically significant(P<0.05), There was no difference in the content of LDL in the group with subclinical hyporthyroidism compared with the control(P>0.05). But the LDLwas increased obviously in the group with subclinical hyporthyroidism compared with the subclinical hyperth- yroidism(P<0.05).3 Resistin was increased in the group of subclinical hyperthyroidism compared with the control and there was significant difference(P<0.05),The HCYand URIC was decreased obviously and the difference was statistically significant(P<0.01) There was no difference in the content of Vit B12 and Folate in the group with subclinical hyperthyroidism compared with the control(P>0.05). The serum resistin and folate were decreased in the group of subclinical hypothyroidism compared with the control and the difference was statistically significant(P<0.05),while the HCY was increased obviously and the difference was statistically significant(P<0.01).4 There was negative correlation between the level of TSH and resistin in the group of subclinical hyperthyroidism and the group of subclinical hypothyroidism(r=-0.516 P<0.01;r=-0.411 P<0.05).There was positive corr- elation between the level of TSH and HCY in the group of subclinical hyperthyroidism and the group of subclinical hypothyroidism(r=0.495 P<0.01; r =0.441 P<0.01).Conclusion:The change of resistin uric and HCY perhaps have roles on the diagnosis of subclinical thyroid dysfunction, the therapeutic effect and estimate the occurrence and development of disease. This study was to provide experimental basis for preventing the incidence of complications of subclinical thyroid dysfunction.