| Objective: Through the study of the correlation between chronic atrophic gastritis with intestinal metaplasia about turbidity toxin and pathological histology, serum levels of IL-6, the expression of Stat3, NF-кB in gastric mucosa, we try to discuss the relativity between turbidity toxin intrinsic card and pathology, IL-6, Stat3 and NF-кB, and to provide the basis for the theory of turbidity toxin in the clinical application of chronic atrophic gastritis with intestinal metaplasia.Methods:1 According to accessing to relevant literature,referring to the related standard of diagnosis and consulting professional experts, a clinical questionnaire about chronic atrophic gastritis with intestinal metaplasia was made. 2 Determine the study sample size, which refers to the number of cases needed for the research, mainly through formula to calculate, and the number of cases with reference to related research of the same kind, then it was decided that the number of cases for this research was 86. 3 The research objects were patients in chronic atrophic gastritis with intestinal metaplasia diagnosed after the gastroscope and pathological examination, meet the inclusion criteria, and meet the research requirement by the TCM four diagnostic methods, who visited the Chinese medicine hospital in Hebei province from 2012 December to 2014 October. Collect the patients’ age, gender, family history, course of diseases, clinical symptoms, gastroscopy and pathological manifestations, and other relevant information to fill in the questionnaire and synthetic quantified according to the related standard. 4 Collect the patients’ serum to detect the concentration of the IL- 6 in each sample with double antibody sandwich ELISA method. 5 Choose the gastric mucosal tissue with standard of research, and record the microscopically. Part of tissue sections were stained with HE to record the histopathology, and the residual tissue sections were detected the expression of Stat3 and NF-кB by immunohistochemical SP method. 6 According to diagnostic criteria of traditional Chinese medicine with dialectical analysis of clinic patients, the satisfactory patients were divided into two groups, including turbidity toxin intrinsic group of 57 cases, and liver stomach with group of 29 cases. According to western medicine diagnostic criteria, the satisfactory patients were divided into three groups, including mild intestinal metaplasia group of 38 cases, moderate intestinal metaplasia group of 28 cases,and severe intestinal metaplasia group of 20 cases. Then the results were statistically analyzed.Result:1 Compare turbidity toxin intrinsic syndrome group with liver stomach with group 1.1 Compare the gastroscope performance(Table 3,Fig.2) Gastroscope performance of two groups were common for chronic gastritis, erosion, shrinkage,etc.There was no statistically significant difference(P>0.05). 1.2 Compare the degree of gastric mucosa pathological with intestinal metaplasia(Table 4) In the group of turbidity toxin intrinsic(57cases), mild intestinal metaplasia group was 20 cases, moderate intestinal metaplasia group was 19 cases, and severe intestinal metaplasia group was 18 cases. In liver stomach with group, mild intestinal metaplasia group was 18 cases, moderate intestinal metaplasia group was 9 cases,and severe intestinal metaplasia group was 2 cases. The degree of gastric mucosa intestinal metaplasia in turbidity toxin intrinsic group was higher than liver stomach with group,and the difference was statistically significant(P < 0.05). 1.3 Compared the serum levels of IL-6(Table 5) There was no difference in the serum levels of IL-6 between turbidity toxin intrinsic group and liver stomach with group. There was no statistically significant difference(P>0.05). 1.4 Compared the expression of Stat3 and NF-кB in gastric mucosa(Table 6) The expression of Stat3 and NF-кB in gastric mucosa in turbidity toxin intrinsic group was higher than liver stomach with group,and the difference was statistically significant(P < 0.05). 2 Compared different degree of intestinal metaplasia groups 2.1 Compared with the serum levels of IL-6(Table 7) The serum levels of IL-6 in severe intestinal metaplasia was higher than the mild intestinal metaplasia group, there was statistically significant difference(P<0.05). The serum levels of IL-6 in moderate intestinal metaplasia was higher than the mild intestinal metaplasia group, there was statistically significant difference(P<0.05). The serum levels of IL-6 in severe intestinal metaplasia was not different from moderate intestinal metaplasia group, there was no statistically significant difference(P>0.05). 2.2 Compared with the expression of Stat3 and NF-кB in gastric mucosa(Table 8) The expression of Stat3 and NF-кB in the group of severe intestinal metaplasia was higher quantity than the mild intestinal metaplasia group, there was statistically significant difference(P<0.05). The expression of Stat3 and NF-кB in the group of moderate intestinal metaplasia was higher quantity than the mild intestinal metaplasia group,there was statistically significant difference(P<0.05).The expression of NF-кB in the group of severe intestinal metaplasia was higher quantity than the moderate intestinal metaplasia group, there was statistically significant difference(P<0.05).There was no difference in the expression of Stat3 between moderate intestinal metaplasia and mild intestinal metaplasia group,there was no statistically significant difference(P>0.05).Conclusions:1 There is a definite relationship between turbidity toxin intrinsic and the degree of intestinal metaplasia. 2 The changes of serum levels of IL-6 is positively correlated with the degree of intestinal metaplasia.It can speculate that the serum levels of IL-6 degree increased with the increasing trend of intestinal metaplasia degree. 3 The expression of Stat3 and NF-кB in gastric mucosa increases with the increasing trend of intestinal metaplasia degree.And it can speculate that Stat3 and NF-кB are involved in the pathogenesis of intestinal metaplasia in gastric mucosa. |
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