| Fibroblast growth factor homologous factors,namely FHFs, which includes FHF1(FGF12)、FHF2(FGF13)、FHF3(FGF11)、FHF4(FGF12), is a special subfamily of Fibroblast Growth Factor(FGF). It can combine with sodium channel and affect the expression and function of the channels. So the study of FHFs expression in human atrial and ventricular muscle tissue has important physiological significance.In this experiment, we detected the expression characteristics of the fibroblast growth factor homologous factors(FHFs) subtypes of m RNA in human atrial and ventricular muscle tissue using Real-Time q PCR.Objective: To establish a reliable real-time RT-PCR method for detecting FHF m RNA expression in human atrial and ventricular muscle tissue. To detect the expression characteristics of the fibroblast growth factor homologous factor(FHF) in human atrial and ventricular muscle tissue.Methods:Take normal human atrial and ventricular muscle tissuer from patients and clearage heart tissue by Trizol to get m RNA. RNA was extracted from human atrial and ventricula myocytes and. c DNA was made by reverse transcription Polymerase Chain Reaction(RT-PCR). Using c DNA as template,Real time quantitative PCR(QPCR) was employed to amplify and quantify relative expression levels of FHFs isoforms in human atrial and ventricula myocytes. Finaly, agarose gel electrophoresis technology was used to visalize to PCR products.Result:In the norma human atrial muscle tissue, the expression of FGF12 B is the most obvious, one while other isoforms are only 4% or even lower; in normal ventricular muscle, to express the dominant FGF12 B, the expression of FGF13VY/Y was about 26.7±9% of FGF12 B, the other subtypes are expressed very low.Conclusion:In atrial and ventricular muscle tissue, the expression of FGF12 B is much higher than all the other subfamily of FHFs, suggested that FHFs might have important physiological significance in human heart. |
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