| Dendrimer has a high compact and uniform shape, it is thought to be a promising candidate in surface modification due to its unique architecture and plurifunctionlity. In some research area, scientists could precisely control the size, molecular weight as well as chemical functional groups of dendrimers through choosing the synthetic method. In this paper, we used a divergent method to prepare the amino/hydroxyl-terminated dendrimers on titanium oxide thin flims. As a result, the density of functional groups on TiO2 flim augments greatly. Moreover, the biocompatibility of this kind of material surface was investigated.In this experiment, TiO2 film was prepared on Si (100) wafer by unbalanced magnetron sputtering. Then 4 generations of amino-terminated dendron were formed through Michael addition and amidation reaction. The amino-terminated dendritimers were indicated by Fourier transform Inrfared Spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. Furthermore, in vitro platelet adhesion and aggregation assay, endothelial cells(EC) cluture were taken to observed the biocompatibility of this amino-terminated dendrimers on titanium oxide thin film.In addition,11-Hydroxyundecylphosphonic acid was synthesized to form a phosphonic interface on TiO2 film. Then 4 generations of hydroxyl-terminal dendrons were prepared, which were certificated by FTIR and SEM. Next we investigate its surface characteristics with regard to haemocompatibility.In conclusion, this paper inspected and verified a modified surface with dendrimers which could effectively inhibit platelet adhesion and activation, benefit to EC adhesion and proliferation. On the other hand, the number of surface functional groups on TiO2 film augments greatly, this modification offers more opportunities to graft other biomolecule further. |
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