| ObjectiveTo investigate changes of intestinal flora and correlation with cytokines and liver function in patients with chronic hepatitis B and liver cirrhosis.MethodsIn this study, 50 patients with chronic hepatitis B and 50 patients with cirrhosis were selected from the Department of infectious diseases of the First Affiliated Hospital of Anhui Medical University, 30 healthy subjects were selected from Health Examination of the First Affiliated Hospital of Anhui Medical University. Nine kinds of intestinall flora were quantified by q PCR to compare the changes between three groups. Then the IL-17 A, IL-1β, IL-6, IFN-α, CXCL-13 concentrations in the serum of patients with chronic hepatitis B were measured with ELISA to explore the relationship between intestinal flora and cytokines, liver function.Results1. The numbers of Bifidobacterium, Lactobacillus, Bacteroides and Ruminococcus were significantly decreased in patients with chronic hepatitis B and liver cirrhosis compared with healthy controls, while the numbers of Enterobacteriaceae, Enterococcus, Clostridium, Candida albicans and Prevotella were significantly increased.2. Enterococcus was positively linked to IL-17 A in patients with chronic hepatitis B. IL-17 A levels were negatively correlated with TBIL, while without correlation with ALT, AST and HBVDNA.3. Positive correlations were found between Enterobacteriaceae and PT, as well as Enterococcus and ALT, AST in patients with chronic hepatitis B. Bifidobacterium was negatively linked to AST, AKP, HBV DNA. Prevotella was negatively linked to AST, AKP, PT. There was a negative correlation between Ruminococcus and ALB, as well as Bacteroides and AST, PT.ConclusionVarying degrees of intestinal flora imbalance exists in patients with chronic hepatitis B and liver cirrhosis. The overgrowth of Enterococcus acts synergistically with IL-17 A to induce liver inflammation and result in impaired liver function. |
PDF Full Text Request