Impact Of Smoking On Nontesticular Germ Cells, Testosterone Levels And Sperm Counts Of Male Mice

Methods:Fifty male mice were divided randomly into five groups:After treated with cigarette smoking concentration for 6 weeks,12 weeks,without special treatment of the control group mice and quited smoking 6 weeks.Five groups of mice testis tissues were stained with HE staining,the morphologic and structural change of leydig cells and sertoli cells were observed under lightmicroscopy.Immunohistochemistry method was used to count the number of leydig cells and sertoli cells in the five groups.Using enzyme-linked immunosorbent assay to measure the content of testosterone in mice.Detecting the cadmium in serum with graphite furnace atomic absorption spectrometry and counting the number of epididymal sperms.Results:Findings of HE taining show that the number of leydig cells is decreased obviously,the number of leydig cells in mice testis scarce with smoking 12 weeks is rarely or even disappear. While the number and form of the sertoli cells have no obvious change compared with the control groups.Results of Immunohistochemistry show that the number of sertoli cells at smoking groups mice compared with the control groups have no obvious difference(p>0.05).Along with the extension of smoking time,the number of the leydig cells sinificantly decrease(p<0.01).The level of serum testosterone in the smoking groups were sinificantly lower than the control groups(p<0.01).In addition,it was postively correlated with the smoking time.But three sets of date in quited smoking group were higher than the smoking groups.Conclusion:Cigarette smoking can lead to the destruction of the leydig cells,influence the generation of testosterone,and resulting in spermatogenic obstacles.While quited smoking can reverse the phenomenon.