The New Vaccine Adjuvants Effect Research On Chito-oligosaccharides And Creatine Phosphate Sodium

Adjuvant can enhance the body’s immune response level nonspecificly and new vaccines make adjuvant use more widely, but most vaccines still use aluminun adjuvant as their main adjuvant, so the new adjuvant need to be developed.Based on biological activities of chito-oliogochitosan and danger signals mode theory, we tried to use chito-oligosaccharides and creatine phosphate sodium as new vaccine adjuvants respectively. In this study, we investigate the adjuvant effection on humoral immune response induced by hepatitis A virus(HAV) in mice,. The results show that, (1)the anti-HAV IgG antibody level of mice in COS 2.5mg group, COS 5mg group and COS lOmg group are significantly higher than antigen control group in various time of the experiment. The antibody level of COS 2.5mg group and COS 5mg group are sigificantly higher than aluminium adjuvant control at the 4th,8 th and 12 th week (P<0.05); the antibody level of COS 10mg group are significantly higher than aluminium adjuvant control at the 8 th week and 12 th week (P<0.05); the antibody level of COS 2.5mg,COS 5mg and COS 10mg groups still equal to aluminium adjuvant group until the 16th week; the optimal dosage of COS in this experiment is 5mg. (2) The anti-HAV IgG antibody level in mice of all the Creatine Phosphate Sodium groups are significantly higher than antigen control at the 4th,8th and 12th week. The antibody level of CP 5mg group is significantly higher than aluminun adjuvant group at the 8week (P<0.05); The optimal dosage of CP in this experiment is 5mg. In adjuvant safety testing assay, we find COS and CP as adjuvant are all have good security and no obvious toxicity during the experiment.Conclusion:Chito-oligosaccharides and creatine phosphate sodium can effectively enhance the humoral immune response level induced by HAV in mice, and sometimes the anti-HAV IgG antibody induced by chito-oligosaccharides even higher than aluminiun adjuvant in high-dosage groups. Our study provides that chito-oligosaccharides and creatine phosphate sodium can develop to new vaccine adjuvants.