| ObjectivesEnvironmental problem becomes increasingly severe, along with which, skin photoaing disease becomes more and more common. In epidemiological survey, we found that nearly 80% of skin aging disease belongs to photoaging which will cause coarse and sagging skin, even more, cause skin disorders such as actinic purpura and skin cancer. Such skin diseases will not only ravish our body, torture our mind as well as make us lose confidence, but also bring negative effect to our daily job. Hence, how to inhibit the UV deleterious effects is never off the table and developing an anti-aging medicine is in demand.In our previous investigation on Lingnan genuine medicinal herb Pogostemon cablin (Blanco) Benth., its essential oil was demonstrated to has the skin protection effects. And its principal ingredient--patchouli alcohol (PA) was verified to effectively inhibit inflammatory response. Moreover, the tricyclic sesquiterpene pharmaceutical structure of PA also implied that PA may possess antioxidation effect. Accordingly, we put the antioxidant and anti-inflammatory paths as the breakthrough point and applicated with biochemical detection and pathological examination method to investigate inhibitory effect and functional mechanism of PA on the mice skin photoaging so that we can provide basic research data for developing anti-photoagig leading compounds which using tricyclic sesquiterpene structure as the basic parent structure.Methods1. The anti-photoaging effect of PAIn this research, the long-wave ultraviolet (UVA) combined with medium wave ultraviolet (UVB) were used as the simulated solar resource to irradiate the KM mice to build the photoaging model. At the same time, the dorsal skin of mice were topically treated with Patchouli alcohol (PA) and the effectiveness of this natural compound was evaluated by macroscopic evaluation and the pinch test of the skin.2. The mechanism investigation of the anti-photoaging effect of PA In order to further investigate the underlying mechanism of the protective effects of PA on photoaging mice, the activities of antioxidant enzymes (SOD, GSH-Px) and the content determination of MDA, hydroxyproline (Hyp) were tested using biochemical detection method. The content of inflammatory factors such as IL-6, IL-10, TNF-α and the matrix metalloproteinases (MMP) such as MMP-1 and MMP-3 were also detected by ELISA method. Further, H&E staining and Gomori’s aldehyde fuchsin staining of the skin tissues were performed to evaluated the situation of skin structure.ResuIts1. The anti-photoaging effect of PAIn this part, photoaged mice were topically applicated with PA (0.6mg/mice, 0.12mg/mice,0.24mg/mice), results demonstrated for the first time that PA could reverse the UV-induced photoaged skin characteristic such as coarse, leather like appearance, nodules and sun burn, especially for middle and high dose groups. Specially, statistic results showed that, compared with vehicle group, PA could slow down the rising velocity of the macroscopic evaluation score and improved the skin recovery rate significantly.2. The mechanism investigation of the anti-photoaging effect of PAThe current study results of this part elucidated that PA treament (0.6mg/mice,0.12mg/mice,0.24mg/mice) could obviously elevated the activities of SOD and the high dose of PA could increase the activities of GSH-Px. The MDA content was decreased by about 30%. Further, in the high dose group, content of collagen was increased. In the aspect of anti-inflammation, middle and high dose group have showed a decline in the contents of IL-6, IL-10 and TNF-α, though the low dose of PA just decrease the content of TNF-a. At the same time, the high dose group of PA have inhibited the secretion of MMP-1 and MMP-3.ConelusionIn this study, a internationally accepted in vivo model of the photoaging was used to investigate the skin protective effect of PA. Evidence was presented in this study that PA dramatically suppressed the UV-induced overproduction of MMP-1 and MMP-3 through enhancing the activities of antioxidant enzymes and inhibiting the production of inflammatory markers, which finally reversed the UV-mediated collagen and matrix destruction. We believe that PA has a strong potential to be developed into a therapeutic and cosmetic product against photoaging. |
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