MicroRNA-27a Regulates The Maturation Process And Cytokine In LPS-stimulated Dendritic Cells

Objective To explore the effects of micro RNA-27a(mi R-27a) on the phenotype and cytokine secretion in LPS-stimulated dendritic cells.Method Murine bone marrow-derived dendritic cells were transfected with mi R-27 a mimics, mi R-27 a inhibitors and negative control mimics, and then stimulated by LPS for 24 hours. Dendritic cells exposed to LPS were collected for analysis of the DC immunophenotype by flow cytometry and supernatants were collected to determine cytokine lever. Moreover, the capability of stimulating allogeneic CD4+T cell proliferation was measured by MLR( mixed lymphocyte reaction).Results Compared with im DC, LPS-stimulated dendritic cells expressed significantly higher levels of MHCII and co-stimulatory molecules such as CD80 and CD86(P <0.001). Compared with negative mimics and mi R-27 a inhibitors, DC transfected with mi R-27 a mimics expressed lower levels of MHCII and co-stimulatory molecules such as CD80 and CD86(P< 0.001). For cytokine secretion,mi R-27 a mimics enhanced IL-10 production(P< 0.01) and reduced the production of IL-12(P< 0.05). For MLR, mi R-27 a mimics suppressed allogeneic CD4+T cell proliferation.Conclusion Mi R-27 a may regulate the maturation process and cytokine secretion in LPS-stimulated dendritic cells.