Role Of Aberrant Expressed Long Non-coding Rna In Glioma

Gliomas are the most frequency primary tumors in the central nervous system of adults. Surgery, radiation, and chemotherapy are still the main therapeutic strategies, but patients with malignant gliomas remain in grim condition. Recent studies have identified a class of long non-coding RNA molecules, named lncRNA with longer than 200 nucleotides, which were reported to participate in regulating the development of various diseases including gliomas. With lncRNA expression profiling microarrays,some aberrant expression of long non-coding RNA have been found in previous study,such as ak125809, ak098473, uc002 ehu.1, bc043564, NR₀27322,UBE2CP3-001,loc285194.Some reports have previously shown LncRNA loc285194 was within a tumor suppressor unit in osteosarcoma and to suppress tumor cell growth. However, there were no studies of lncRNA loc28194 in gliomas.To investigate the lncRNA loc285194 expression levels in glioma and the role of lncRNA loc285194 in human glioma u87 cell biological functions,Methods quantitative real-time polymerase chain reactions for lncRNA-loc285194 expression were performed in 4 epilepsy brain tissues and 40 pairs of glioma tissues and adjacent normal tissues in our department. Cell growth, and apoptosis were respectively tested by CCK8 assay and flow cytometry. Western blot was also used to detected p53 protein. Result: lncRNA loc285194 was significantly low-expressed in glioma tissues. Over-expressed lncRNA loc285194 inhabited human glioma U87 cells growth（p<0.05） and promoted U87 cells apoptosis.（p<0.05）Conclusions: lncRNA loc285194 is low-expressed in human glioma tissues.Over-expression lncRNA loc285194 suppress glioma cells growth and promoted glioma cell apoptosis through inducing p53 protein. It might be a new gene therapy target.Moreover, LncRNA UBE2CP3-001 was also found overexpressed in gliomas rather than adjacent normal tissues through lncRNA expression profiling microarrays. It is encoded by the gene UBE2CP3 located at chromosome 4q12.However, the molecular functions of lncRNA UBE2CP3-001 are largely unknown. Compared to lncRNA loc285194, it is more significant. And in the next study we used same way to investigate the role of UBE2CP3-001 in the genesis of glioma. A lentiviral vector which could down-regulate UBE2CP3-001 expression was constructed in this study. Our data showed decreased expression of lncRNA UBE2CP3-001 could suppress cell migration and invasiveness in U87 human glioma cell lines. Low expression of LncRNA UBE2CP3-001 also retarded cell proliferation and promoted cell apoptosis in U87 cells. Furthermore, MMP9 protein and TRAF3IP2 detected were positively correlated with lncRNA UBE2CP3-001 expression.It suggested that lncRNA UBE2CP3-001 had impact on U87 cells growth through inducing TRAF3IP2, an activator of NFkappB/MMP9 signaling pathway, to promote glioma cells growth. Taken together, these data suggest a role of lncRNA UBE2CP3-001 in the molecular etiology of glioma and implicate the potential application of lncRNA UBE2CP3 in glioma therapy.