Expression Of PTP4A1 In Esophageal Squamous Cell Carcinoma And Their Clinical Implications

Objective:To investigate PTP4A1(protein tyrosine phosphatase type IVA, member 1) expressions in human ESCC(esophageal squamous cell carcinoma) tissues and to explore the relationship between PTP4A1 expressions and clinicopathological features of human ESCC.And to evaluate the influence of PTP4A1 expression level in the postoperatives with ESCC.Methods:Expressions of PTP4A1 in 155 cases of ESCC and 41 cases of normal esophageal mucosa and 41 cases of ESCC adjacent tissues were examined by immunohistoehemistry on TMAs.All these cases have complete clinical data and follow-up data.The relationship between PTP4A1 expressions and clinicopathological features of human ESCC were analysed by X2 test and Correlation analysis.The analysis of the distribution of PTP4A1 in both OS and TTR of the postoperatives with ESCC was performed by Kaplan-Meier and Cox Regression method.Results:1 The results of the immunohistoehemistry showed that over-expression of PTP4A1 was located in the cytoplasm and cell membrane of human ESCC.And PTP4A1 expression was significantly Higher in ESCC tissue than normal esophageal mucosa(P=0.01).There was no obvious difference between ESCC tissue and ESCC adjacent tissues(P=0.085),normal esophageal mucosa and ESCC adjacent tissues(P=0.148) in the expressions of PTP4A1.2 Expression of PTP4A1 in ESCC tissue was closely related with lymphatic metastases(P=0.008<0.05),TNM stage(P=0.04),Invasion depth(P=0.005),Tumor differentiation(P=0.032),but had no relationship with age,gender,History of smoking, drinking and family history of cancer.3 Univariate analysis was calculated by the Kaplan-Meier method(the log-rank test).Then,the results showed that over-expression of PTP4A1 in tumor,lymphatic metastases,TNM stage,Invasion depth,Tumor differentiation, History of drinking were the risk factors that affect the survival of the postoperatives with ESCC.And over-expression of PTP4A1 in tumor, lymphatic metastases,TNM stage,Invasion depth,Tumor differentiation were the risk factors of the recurrence.The survival of the over-expression of PTP4A1 group was significantly lower than the the lower expression group(P=0.022<0.05),and the TTR was obvious worse than the the lower expression group(P=0.005<0.05).4 Multivariate analysis was done using the Cox multivariate Proportional hazards regression model with stepwise manner(forward,likelihood ratio).The over-expression of PTP4A1 in ESCC tissue was an independent risk predictive indicator for survival(HR=4.258,95%CI for Exp(B):1.082-4.275,P=0.029)and recurrence(HR=2.190,95%CI for Exp(B):1.035-4.635 P=0.040)lymphatic metastases(HR=2.150,95%CIfor Exp(B):2.161-8.390,P<0.001) and without reasonable postoperative adjuvant chemotherapy( HR=3.002,95%CI for Exp(B):1.517-5.940,P=0.002)were an independent risk predictive indicator for survival.5 ROC Curve was taken to analyze the prediction efficiency of expression of PTP4A1 in tumor.The results showed that expression of PTP4A1 in tumor has some predictive value on overall survival(AUC=0.600)and recurrence rates(AUC=0.601).Conclusions:1 The results of PTP4A1 expression was significantly higher in ESCC tissue than normal esophageal mucosa,expression of PTP4A1 in ESCC tissue was closely related with lymphatic metastases,TNM stage,Invasion depth and Tumor differentiation showed that PTP4A1 may be involved in promoting the occurrence,invasion and metastasis of ESCC.2 The over-expression of PTP4A1 in ESCC cells was an independent risk factor for postoperative recurrence and survival, and may serve as an important role in the metastasis and recurrence ESCC cells. These results suggest that the over-expression of PTP4A1 may serve as the biomarkers in prediction of metastatic potential of ESCC, and may serves as the potential targets for the gene therapy of ESCC,especially the metastatic ESCC,in future. 3.Lymphatic metastases and without reasonable postoperative adjuvant chemotherapy were independent risk predictive indicator for survival. So,these postoperative patients with lymphatic metastases should be strengthened monitoring.And reasonable postoperative adjuvant chemotherapy is helpful to improve the overall survival rate of postoperative patients.