Immunomodulatory Effect Of The Active Ingredients Of Traditional Chinese Medicine Based On The Compatibility Mechanism On Early Esophageal Dysplasia

Objective: This paper intends to study the reversal effect of “Lian huashen jia”formula made by “Forsythia Lignans, Trichosanthin, CortexPeriplocae triterpenodis and dangshen glycoprotein” on different stages ofearly esophageal dysplasia and the potential mechanisms of immunefunction. The animal model of esophageal lesions were induced by chemicalcarcinogen 4- nitroquinoline-1-oxide(4-nitro-quinoline- 1-oxide, 4NQO) inC57 BL / 6 mice.Method:1 300(150 male and 150 female) mice used in the experiment weredivided into six groups at random. Group A1(normal group),Group A2(methylcellulose control group),Group B(induced cancer group),Group C(treatmentgroup),Group D(prevention group),Group E(all-trans-retinoic acid,ATRApositive control group).30 mice were involved in A1 and A2, 60 mice wereinvolved in B,C,D and E. The 4NQO solution,dissolved in sterile water at aconcentration 100μg/ml,was given as a drinking water to B, C, D and E mice.“Lian hua shen jia”formula made by "Forsythia Lignans, Trichosanthin,Cortex Periplocae triterpenodis and dangshen glycoprotein and dissolved in1.5% methyl cellulose according to 2mmg: 1.4mg: 1.4mg: 0.7mg mixingproportions. Mice in group D were given with “Lian hua shen jia”formula bygastric from the beginning of the experiment.At 15 th week mice in group Cwere given with “Lian hua shen jia”formula by gastric. Because mice ingroup B appeard atypical hyperplasia. Mice in group E were given with“all-trans retinoic acid”(ATRA) by gastric at the same time with C. Mice ingroup C, D and E were given medice by gastric,0.1ml/time,three times a week.2 Mice in A1, A2, B and D were killed every group at 12 th week, andobserved whether esophageal dyslasia. At 15 th week,mice in these group weredealt with the same method, and observed esophageal dyslasia. Andthen,4NQO which drinked by mice was replaced with sterile water. Mice ingroup A1 and group A2 were killed five each group and in group B,C,D and Ewere killed fifteen every group at 12 th week. At 15 th week mice in these groupwere dealt with the same method. The rest of the mice were killed at 24 thweek.3 We established the mouse model of the esophageal precancerouslesions and observed the different of esophagus with HE in B and identifiedthe tumor progression4 We extract mice spleen and observed the effect of “Lian huan shen”formula on lymphocytes in spleen cells. CD4+T,CD8+,B7-H4 and CD4+CD25+foxp3+T cells in mice spleen were detected by Flow Cytometry at 18 thweek,21th week and 24 th week. We observed the changes of the surfacemolecules.Results:1 we established early esophageal dysplasia in mice model successfully.We found that group B induced by 4NQO appeard mild dysplasia by H&Estaining at 15 th week2 HE staining results showed that esophageal tissue lesion in mice wasthere are significant difference at 24 th week, P<0.05, cancer rate of C, D, E issignificantly lower than group B, P<0.05, It was showed that“Lian hua shenjia”formula can slow down the progression of esophageal lesions.3 The experimental results show that CD4 + CD25 + foxp3 + regulatory Tcell in spleen lymphocytes at 18 th week and 21 th week were no significantdifference,(P>0.05)but at 24 th week there were significant differences(P<0.05).4 The expression of B7-H4 on CD4+T cells was significant difference at21 th week, P<0.05.The expression rate of B7-H4 in CD8+T and CD4+CD25+Tfoxp3+T cells were no significant difference at 18 th week, 21 th week and 24 thweek, P<0.05.Conclusion:1 we established early esophageal dysplasia in mice model successfully.2 “Lian hua shen”formular have a good improvement in esophagealprecancerous,and it can improve immune function.